Exploration of obese-related genes in cats and dogs.

• Project/Area Number: 24580467
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Nippon Veterinary and Life Science University
• Principal Investigator: ISHIOKA Katsumi 日本獣医生命科学大学, 獣医学部, 准教授 (60409258)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 肥満 / 犬 / 遺伝子多型 / SNP / 猫 / 一塩基多型 / GPR120 / β3アドレナリン受容体

Outline of Final Research Achievements

Obesity is a clinical problem in dogs. We explored genetic variants using genomic DNA samples collected from 141 dogs. We found 5 synonymous and 7 non-synonymous variants in the canine beta-3 adrenergic receptor gene, in which c.749C>T (p.Ser250Phe) was found more in obese dogs and c.1121C>G (p3Pro374Arg) was found more in lean dogs. We found 5 synonymous and 4 non-synonymous variants in the canine GPR120 gene, in which c.595C>A (p.Pro199Thr) was found more in obese dogs. The variant c.595C>A (p.Pro199Thr) was found only in beagles. These findings will be applied to clinical nutrition in dogs.

Research Products

• [Journal Article] Genetic variants of the unsaturated fatty acid receptor GPR120 relating to obesity in dogs. (2015)
  • Author(s): Masahiro MIYABE, Azusa GIN, Eri ONOZAWA, Mana DAIMON, Hana YAMADA, Hitomi ODA, Akihiro MORI, Yutaka MOMOTA, Daigo AZAKAMI, Ichiro YAMAMOTO, Mariko MOCHIZUKI, Toshinori SAKO, Katsutoshi TAMURA and Katsumi ISHIOKA
  • Journal Title: Journal of Veterinary Medical Science Volume: -
  • NAID: 130005107589
  • Peer Reviewed
• [Presentation] イヌβ3アドレナリン受容体遺伝子のC749T多型と体重変動 (2013)
  • Author(s): 宮部真裕、小野澤栄里、百田豊、呰上大吾、左向敏紀、石岡克己
  • Organizer: 日本ペット栄養学会第15回大会
  • Place of Presentation: 東京
• [Presentation] イヌGPR120遺伝子の一塩基多型解析と犬種 (2013)
  • Author(s): 宮部真裕、大門愛、山田華、小野澤栄里、百田豊、呰上大吾、石岡克己
  • Organizer: 日本獣医学会学術集会第156回大会
  • Place of Presentation: 岐阜
• [Presentation] イヌにおけるβ3アドレナリン受容体（β3AR）遺伝子のSNP解析
  • Author(s): 宮部真裕、阿部真由美、小野澤栄里、百田豊、呰上大吾、石岡克己
  • Organizer: 日本獣医学会第154回大会
  • Place of Presentation: 岩手
• [Presentation] 肥満の病態とアディポカイン
  • Author(s): 石岡克己
  • Organizer: 第27回MMIシンポジウム
  • Place of Presentation: 横浜
  • Invited