| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
We demonstrated the molecular breedings of lactate-producing fungi in Phanerochaete sordida YK-624. Lactate dehydrogenase (LDH) gene of Bifidobacterium longum was introduced in P. sordida YK-624, and 0.690 g/L lactate was detected in culture fluid inoculated with the most producing transformant. To improve the production of lactate, we constracted pyruvate decarboxylase gene-knock down plasmid. Both the LDH expression plasmid and the knock down plasmid were co-transformed into P. sordida YK-624, and 0.922 g/L lactate was detected in culture fluid inoculated with the most producing transformant. Moreover, we developed the bioreactor which lignin was effectively degraded by white-rot fungi under solid state fermentation.
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