Development of lactate-producing white-rot fungi from woody biomass
Project/Area Number |
24580475
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Boundary agriculture
|
Research Institution | Shizuoka University |
Principal Investigator |
HIRAI Hirofumi 静岡大学, (連合)農学研究科(研究院), 教授 (70322138)
|
Co-Investigator(Kenkyū-buntansha) |
KIMBARA Kazuhide 静岡大学, 工学部, 教授 (30225122)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | 乳酸発酵 / 白色腐朽菌 / バイオリアクター / 木質バイオリファイナリー |
Outline of Final Research Achievements |
We demonstrated the molecular breedings of lactate-producing fungi in Phanerochaete sordida YK-624. Lactate dehydrogenase (LDH) gene of Bifidobacterium longum was introduced in P. sordida YK-624, and 0.690 g/L lactate was detected in culture fluid inoculated with the most producing transformant. To improve the production of lactate, we constracted pyruvate decarboxylase gene-knock down plasmid. Both the LDH expression plasmid and the knock down plasmid were co-transformed into P. sordida YK-624, and 0.922 g/L lactate was detected in culture fluid inoculated with the most producing transformant. Moreover, we developed the bioreactor which lignin was effectively degraded by white-rot fungi under solid state fermentation.
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Report
(4 results)
Research Products
(5 results)