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Substrate binding affinities of catalytic modules from cellulases

Research Project

Project/Area Number 24580476
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Boundary agriculture
Research InstitutionMie University

Principal Investigator

KARITA Shuichi  三重大学, 地域イノベーション学研究科, 教授 (90233999)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Keywordsセルラーゼ / バイオマス / 糖化 / 基質親和性 / 触媒モジュール / 基質相互作用 / 酵素活性 / エンドグカナーゼ / 糖質加水分解酵素 / 加水分解酵素ファミリー / 基質結合 / エンドグルカナーゼ / 等温滴定熱量計 / 触媒親和力
Outline of Final Research Achievements

Microorganisms produce multiple cellulases for degradation of biomass. Substrate affinities of these cellulolytic enzymes were examined. Three catalytic modules of enzymes, Cel5A, Cel5B, and Cel5E from Clostridium thermocellum were mutated to lose catalytic activities. Mutated proteins were tested for binding affinities of cellulosic substrates. Binding capacity to acid-swollen cellulose was Cel5B > Cel5A > Cel5E, whereas that to ball-milled cellulose was Cel5E>Cel5A>Cel5B. These enzymes are endoglucanases, however, substrate affinities of these catalytic modules depend on morphology of cellulosic substrates.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (5 results)

All 2015 2014 Other

All Presentation (3 results) Remarks (2 results)

  • [Presentation] Clostridium thermocellum由来の糖質加水分解酵素GH5の触媒モジュールの基質結合特性2015

    • Author(s)
      並河有紀、苅田修一、須藤真子、市川俊輔
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 糖質加水分解酵素ファミリー5セルラーゼの触媒モジュールは異なる基質親和性をもつ2014

    • Author(s)
      並河有紀、苅田修一
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜パシフィコ
    • Year and Date
      2014-11-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] GH5セルラーゼの触媒モジュールは異なる基質親和性をもつ2014

    • Author(s)
      並河有紀、市川俊輔、苅田修一
    • Organizer
      第28回セルラーゼ研究会
    • Place of Presentation
      千葉県習志野研修センター
    • Year and Date
      2014-07-11 – 2014-07-12
    • Related Report
      2014 Annual Research Report
  • [Remarks] セルラーゼの話題

    • URL

      http://www.bio.mie-u.ac.jp/~karita/sub3.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] セルラーゼの話題

    • URL

      http://www.bio.mie-u.ac.jp/~karita/sub3.html

    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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