| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Outline of Final Research Achievements |
Microorganisms produce multiple cellulases for degradation of biomass. Substrate affinities of these cellulolytic enzymes were examined. Three catalytic modules of enzymes, Cel5A, Cel5B, and Cel5E from Clostridium thermocellum were mutated to lose catalytic activities. Mutated proteins were tested for binding affinities of cellulosic substrates. Binding capacity to acid-swollen cellulose was Cel5B > Cel5A > Cel5E, whereas that to ball-milled cellulose was Cel5E>Cel5A>Cel5B. These enzymes are endoglucanases, however, substrate affinities of these catalytic modules depend on morphology of cellulosic substrates.
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