Identification of genes responsible for heat and salinity stress tolerances via FOX hunting among collection of E. salsugineum cDNAs
Project/Area Number |
24580483
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Boundary agriculture
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Research Institution | Tokyo University of Agriculture |
Principal Investigator |
TAJI Teruaki 東京農業大学, 応用生物科学部, 准教授 (60360583)
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Research Collaborator |
HIGASHI Yukari
ARIGA Hirotaka
SASO Yasutaka
NAGASHIMA Yuko
FUKUUCHI Takuya
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 塩生植物 / 完全長cDNA / 耐塩性 / 高温耐性 / トランスジェニック植物 / シロイヌナズナ / Thellungiella / Eutrema salsugineum / Thellungiella salsuginea / FOX hunting / Fox hunting |
Outline of Final Research Achievements |
Eutrema salsugineum (also known as Thellungiella salsuginea), a species closely related to Arabidopsis thaliana, shows tolerance not only to salt stress, but also to chilling, freezing, and high temperatures. To identify genes responsible for stress tolerance, we conducted Full-length cDNA Over-eXpressing gene (FOX) hunting among a collection of E. salsugineum cDNAs that were stress-induced according to gene ontology analysis or over-expressed in E. salsugineum compared with A. thaliana. In this study, we identified E. salsugineum HsfA1d (TsHsfA1d) via the FOX hunting as a gene that can confer marked heat tolerance on A. thaliana. Transgenic A. thaliana overexpressing TsHsfA1d showed constitutive up-regulation of many genes in the A. thaliana AtHsfA1 regulon under normal growth temperature. We propose that the products of TsHsfA1d function as positive regulators of A. thaliana heat-stress response and would be useful for the improvement of heat-stress tolerance in other plants.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Group A PP2Cs evolved in land plants as key regulators of intrinsic desiccation tolerance2013
Author(s)
Komatsu K, Suzuki N, Kuwamura M, Nishikawa Y, Nakatani M, Ohtawa H, Takezawa D, Seki M, Tanaka M, Taji T, Hayashi T, Sakata Y
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Journal Title
Nature Communications
Volume: 4
Issue: 1
Pages: 2219-2219
DOI
Related Report
Peer Reviewed
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[Journal Article] Arabidopsis sos1 mutant in a salt-tolerant accession revealed an importance of salt acclimation ability in plant salt tolerance2013
Author(s)
Ariga H, Katori T, Yoshihara R, Hase Y, Nozawa S, Narumi I, Iuchi S, Kobayashi M, Tezuka K, Sakata Y, Hayashi T, Taji T
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Journal Title
Plant Signaling and Behavior
Volume: 8
Issue: 7
Pages: e24779-e24779
DOI
Related Report
Peer Reviewed
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[Journal Article] HsfA1d, a protein identified via FOX hunting using Thellungiella salsuginea cDNAs improves heat tolerance by regulating heat-stress-responsive gene expression.2013
Author(s)
Higashi Y, Ohama N, Ishikawa T, Katori T, Shimura A, Kusakabe K, Yamaguchi-Shinozaki K, Ishida J., Tanaka M, Seki M, Shinozaki K, Sakata Y, Hayashi T, Taji T
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Journal Title
Mol Plant
Volume: 6(2)
Issue: 2
Pages: 411-422
DOI
Related Report
Peer Reviewed
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[Presentation] 耐塩性シロイヌナズナが有する塩馴化能の遺伝学的解析2013
Author(s)
有賀裕剛, 香取拓, Leon Kochian, Owen Hoekenga, Michael A. Gore, 小林佑理子, 井内聖, 小林正智, 坂田洋一, 林隆久, 太治輝昭
Organizer
日本植物生理学会
Place of Presentation
岡山
Related Report
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[Presentation] 耐塩性シロイヌナズナが有する塩馴化能による新奇耐塩性メカニズムの遺伝学的解析2012
Author(s)
有賀裕剛, 香取拓, Leon Kochian, Owen Hoekenga, Michael A. Gore, 小林佑理子, 井内聖, 小林正智, 坂田洋一, 林隆久, 太治輝昭
Organizer
日本植物学会
Place of Presentation
兵庫
Related Report
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