The evaluation of environmental contaminants using human breast carcinoma cells
| Project/Area Number |
24580485
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Boundary agriculture
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| Research Institution | Tokoha University |
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Principal Investigator |
KURUTO Ryoko 常葉大学, 教育学部, 准教授 (50205217)
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| Co-Investigator(Kenkyū-buntansha) |
OHURA Takeshi 名城大学, 農学部, 准教授 (60315851)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | 環境汚染物質 / 多環芳香族炭化水素 / ハロゲン化 / エストロジェン / ゲニステイン / 遺伝子発現 / PCRアレイ |
|---|
| Outline of Final Research Achievements |
A variety of contaminants are present in the environment. Their effects were investigated using human breast carcinoma MCF-7 and MDA-MB-231 cells. The water samples from Tomoe River in Shizuoka city and Manyo pond in Tokoha University showed estrogenic and cytotoxic activity. Then we focused benzo[a]pyrene (BaP), one of polycyclic aromatic hydrocarbons, and its halogenated derivatives, ClBaP and BrBaP. They indicated estrogenic activity. The profile of gene expression of BaP was different from that of the natural estrogenic substances such as estrogen (E2) and genistein (Gen). Their combined effects were examined using MCF-7 cells. Exposure to 10-6 M BaP led to inhibit the proliferative effects of E2. Although 10-5 M BaP itself strongly enhanced cell proliferation, its effect was reduced by the addition of E2. Exposure to ClBaP or BrBaP enhanced the proliferative effects of E2. Gen inhibited the induction of CYP1A1 and IL-6 gene expression by BaP and BrBaP.
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Report
(4 results)
Research Products
(41 results)
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[Journal Article] Spatial and vertical distributions of sedimentary halogenated polycyclic aromatic hydrocarbons in moderately polluted areas of Asia.2015
Author(s)
Ohura, T., Sakakibara, H., Watanabe, I., Shim, W.J., Manage, P.M., Guruge, K.S.
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Journal Title
Environmental Pollution
Volume: 196
Pages: 331-340
DOI
Related Report
Peer Reviewed
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[Journal Article] Sex-dependent difference in the hepatic and pulmonary toxicological effects in mice administrated 7-chlorinated benz[a]anthracene.2014
Author(s)
Sakakibara, H., Ohura, T., Kamiya, Y., Yamanaka, N., Shimada, N., Shimoi, K., Guruge, K.S.
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Journal Title
Fundamental Toxicological Sciences
Volume: 1
Pages: 101-108
Related Report
Peer Reviewed
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[Presentation] Chlorinated Polycyclic Aromatic Hydrocarbons in Air Particulates in North-East Asia.2014
Author(s)
Kakimoto, K., Hagayoshi, H., Takagi, S., Konishi, Y., Kajimura, K., Ohura, T., Hayakawa, K., Toriba, A.
Organizer
International Conference of Asian Environmental Chemistry (ICAEC2014)
Place of Presentation
Bangkok, Thailand
Year and Date
2014-11-24 – 2014-11-26
Related Report
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[Presentation] Atmospheric chlorinated polycyclic aromatic hydrocarbons in East Asia.2013
Author(s)
Kakimoto, K., Nagayoshi, H., Takagi, S., Konishi, Y., Kajimura, K., Ohura, T., Toriba, A., Hayakawa, K.
Organizer
33rd International Symposium on Halogenated Persistent Organic Pollutants and POPs -DIOXIN
Place of Presentation
Korea
Related Report
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[Presentation] Evaluation of toxic equivalents for halogenated polyaromatic hydrocarbons in waste incinerators, Japan.2013
Author(s)
Horii, Y., Minomo, K., Ohtsuka, N., Nojiri, K., Ohura, T., Miyake, Y.
Organizer
33rd International Symposium on Halogenated Persistent Organic Pollutants and POPs -DIOXIN
Place of Presentation
Korea
Related Report
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[Presentation] Organ-specific distribution of 7-chlorinated benz[a]anthracene and regulation of selected cytochrome P450 genes in rats.2013
Author(s)
Ohura, T., Sakakibara, H., Kido, T., Yamanaka, N., Tanimura, N., Shimoi, K., Guruge, K.S.
Organizer
SETAC Europe 23rd Annual Meeting
Place of Presentation
Glasgow, UK
Related Report
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