| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Outline of Final Research Achievements |
A variety of contaminants are present in the environment. Their effects were investigated using human breast carcinoma MCF-7 and MDA-MB-231 cells. The water samples from Tomoe River in Shizuoka city and Manyo pond in Tokoha University showed estrogenic and cytotoxic activity. Then we focused benzo[a]pyrene (BaP), one of polycyclic aromatic hydrocarbons, and its halogenated derivatives, ClBaP and BrBaP. They indicated estrogenic activity. The profile of gene expression of BaP was different from that of the natural estrogenic substances such as estrogen (E2) and genistein (Gen). Their combined effects were examined using MCF-7 cells. Exposure to 10-6 M BaP led to inhibit the proliferative effects of E2. Although 10-5 M BaP itself strongly enhanced cell proliferation, its effect was reduced by the addition of E2. Exposure to ClBaP or BrBaP enhanced the proliferative effects of E2. Gen inhibited the induction of CYP1A1 and IL-6 gene expression by BaP and BrBaP.
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