Study of plant-fiber decomposition by a cellulolytic thermophile for utilization of cellulosic biomass

• Project/Area Number: 24580490
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Boundary agriculture
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: YOKOYAMA Hiroshi 独立行政法人農業・食品産業技術総合研究機構, 畜産草地研究所畜産環境研究領域, 主任研究員 (40391370)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: バイオマス / セルラーゼ / 好熱菌 / C. bescii / マルチドメインセルラーゼ / 繊維分解

Outline of Final Research Achievements

Caldicellulosiruptor bescii (C. bescii) is the most thermophilic bacterium that can degrade crystalline cellulose. Experiments using microcrystalline cellulose and native timothy grass showed that the cellulose system of C. bescii decomposes them more than double as compared to the most common enzyme system prepared from Trichoderma species. A new protein family, PWBP (plant cell wall-binding protein), was identified in the C. bescii cellulose system. The recombinant PWBP49, PWBP57, and PWBP65, prepared from E. coli, showed the highest binding affinities to native plant cell walls among insoluble polysaccharides tested.

Research Products

• [Journal Article] Extracellular secretion of noncatalytic plant cell wall-binding proteins by the cellulolytic thermophile Caldicellulosiruptor bescii (2014)
  • Author(s): Yokoyama H, Yamashita T, Morioka R, Ohmori H
  • Journal Title: J Bacteriol Volume: 196 Issue: 21 Pages: 3784-3792
  • DOI: 10.1128/jb.01897-14
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Crystallization and preliminary X-ray diffraction analysis of the secreted protein Athe_0614 from Caldicellulosiruptor bescii (2013)
  • Author(s): Hiroshi Yokoyama, takahiro Yamashita, Naoki Horikoshi, Hitoshi Kurumizaka, Wataru Kagawa.
  • Journal Title: Acta Crystallographica Section F Volume: 69 Issue: 4 Pages: 438-440
  • DOI: 10.1107/s174430911300554x
  • Peer Reviewed
• [Journal Article] Degradation of microcrystalline cellulose and non-pretreated plant biomass by a cell-free extracellular cellulase/hemicellulase system from the extreme thermophilic bacterium Caldicellulosiruptor bescii (2013)
  • Author(s): Sumiyo Kanafusa-Shinkai, Jun’ichi Wakayama, Kazumi Tsukamoto, Noriko Hayashi, Yasumasa Miyazaki, Hideyuki Ohmori, Kiyoshi Tajima, Hiroshi Yokoyama
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 115 Issue: 1 Pages: 64-70
  • DOI: 10.1016/j.jbiosc.2012.07.019
  • NAID: 110009578192
  • Peer Reviewed