| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Xyloglucan is a majour hemicellulose found in plant cell wall. In this study, various xyloglucan-degrading enzymes were analyzed in detail. Xyloglucanases that cleave β-1,4 glucan backbone chains, and α-xylosidase and β-galactosidase, that cleave the side chain, were cloned, identified and characterized. The analysis of the effect of those enzymes on the degradation of plant cell walls revealed that xyloglucanases showed an additive effect on the cellulosic biomass-degradation of by the cellulases.
Furthermore, by introducing site-directed mutations into the active center of the xyloglucanase it was successfully produced different types of enzymes that have different mode of actions.
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