Analysis of mechanisms for maintenance of cell division activity in plant meristems under stressful conditions

• Project/Area Number: 24580493
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Nagoya University
• Principal Investigator: Takeda Shin 名古屋大学, 生物機能開発利用研究センター, 准教授 (00432253)
• Co-Investigator(Renkei-kenkyūsha): Hattori Tsukaho 名古屋大学, 生物機能開発利用研究センター, 教授 (10164865)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 植物成長 / メリステム / イネ / 環境ストレス / ジャスモン酸 / 塩ストレス / メリステム活性維持 / メリステム活性維持機構

Outline of Final Research Achievements

In plants, maintenance of meristem function is critical to sustain the growth and viability under stressful conditions. We have aimed to understand the underlying mechanisms for such maintenance in rice. Previously, we reported that RSS1 is required for maintenance of cell division activity under stressful conditions. In this research project, we found that recombinant RSS1 protein was less denatured after heat treatment and retained binding activity to the protein phosphatase 1. We also revealed that another factor RSS3, controlling root elongation at the root tip, formed a ternary complex with bHLH and JAZ factors and had a role to repress a portion of JA-inducible genes. Moreover, over-expression of a gene encoding the CYP94C family enzyme alleviated jasmonate responsiveness and resulted in enhanced salt tolerance. The results advocate that regulation of JA responses is important for growth and viability of rice under salinity conditions.

Research Products

• [Int'l Joint Research] Damanhour University(エジプト)
• [Journal Article] Stress-tolerance profiling of a collection of extant salt-tolerant rice varieties and transgenic plants overexpressing abiotic stress tolerance genes (2015)
  • Author(s): Kurotani, K., Yamanaka, K., Toda, Y., Ogawa, D., Tanaka, M., Kozawa, H., Nakamura, H., Hakata, M., Ichikawa, H., Hattori, T. and Takeda S.
  • Journal Title: Plant Cell Physiol. Volume: 56 Issue: 10 Pages: 1867-1876
  • DOI: 10.1093/pcp/pcv106
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Overexpression of a CYP94 family gene CYP94C2b increases internode length and plant height in rice (2015)
  • Author(s): Kurotani, K., Hattori, T. and Takeda S.
  • Journal Title: Plant Signaling & Behavior Volume: 10 Issue: 7 Pages: e1046667-e1046667
  • DOI: 10.1080/15592324.2015.1046667
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Elevated levels of CYP94 family gene expression alleviate the jasmonate response and enhance salt tolerance in rice (2015)
  • Author(s): Kurotani, K., Hayashi, K., Hatanaka, S., Toda, Y., Ogawa, D., Ichikawa, H., Ishimaru, Y., Tashita, R., Suzuki, T., Ueda, M., Hattori, T. and Takeda S.
  • Journal Title: Plant Cell Physiol. Volume: 56 Issue: 4 Pages: 779-789
  • DOI: 10.1093/pcp/pcv006
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Overexpression of the JAZ factors with mutated Jas domains causes pleiotropic defects in rice spikelet development. (2014)
  • Author(s): Hori, Y., Kurotani, K., Toda, Y., Hattori, T., and Takeda, S.
  • Journal Title: Plant Signaling & Behavior. Volume: 9 Issue: 10 Pages: e970414-e970414
  • DOI: 10.4161/15592316.2014.970414
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Life and death under salt stress: same players, different timing? (2014)
  • Author(s): Ismail, A., Takeda, S and Nick, P.
  • Journal Title: J. Exp. Bot. Volume: 65 Issue: 12 Pages: 2963-2979
  • DOI: 10.1093/jxb/eru159
  • Peer Reviewed
• [Journal Article] RICE SALT SENSITIVE3 forms a ternary complex with JAZ and class-C bHLH factors and regulates jasmonate-induced gene expression and root cell elongation (2013)
  • Author(s): Toda Y, Tanaka M, Ogawa D, Kurata K, Kurotani K, Habu Y, Ando T, Sugimoto K, Mitsuda N, Katoh E, Abe K, Miyao A, Hirochika H, Hattori T, Takeda S
  • Journal Title: The Plant Cell Volume: 25 Issue: 5 Pages: 1709-1725
  • DOI: 10.1105/tpc.113.112052
  • Peer Reviewed
• [Journal Article] RICE SALT SENSITIVE3 binding to bHLH and JAZ factors mediates control of cell wall plasticity in the root apex. (2013)
  • Author(s): Toda, Y., Yoshida, M., Hattori, T., and Takeda, S.
  • Journal Title: Plant Signaling & Behavior Volume: 8 Issue: 11 Pages: e26256-e26256
  • DOI: 10.4161/psb.26256
  • Peer Reviewed
• [Journal Article] Molecular characterization of the rice protein RSS1 required for meristematic activity under stressful conditions. (2012)
  • Author(s): Ogawa, D., Morita, H., Hattori, T., and Takeda, S.
  • Journal Title: Plant Physiol. Biochem. Volume: 61 Pages: 54-60
  • DOI: 10.1016/j.plaphy.2012.09.006
  • Peer Reviewed
• [Presentation] イネ小穂の発生を制御する遺伝的機構とジャスモン酸作用 (2016)
  • Author(s): 廣川拓也、佐藤大輔、田中若奈、黒谷賢一、畑中彩希、高牟禮逸朗、武田真、平野博之
  • Organizer: 日本育種学会第129回講演会
  • Place of Presentation: 横浜
  • Year and Date: 2016-03-21
• [Presentation] CYP94ファミリー遺伝子の発現上昇はイネの塩処理条件下での生存能を高める (2015)
  • Author(s): 黒谷賢一、林憲志、畑中彩希、戸田陽介、小川大輔、市川裕章、石丸泰寛、田下諒、鈴木健史、上田実、服部束穂、武田真
  • Organizer: 第56回日本植物生理学会年会（東京）
  • Place of Presentation: 東京
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Jasドメインに変異をもつJAZ因子の発現によるイネ小穂の貫生化の誘発 (2014)
  • Author(s): 堀佑太朗、黒谷賢一、戸田陽介、服部束穂、武田真
  • Organizer: イネ遺伝学・分子生物学ワークショップ2014
  • Place of Presentation: 東京
  • Year and Date: 2014-07-11 – 2014-07-12
• [Presentation] RSS1 required for maintenance of cell proliferation activity under stress conditions ensures G1-S transition in rice. (2012)
  • Author(s): Ogawa, D., Abe, K., Miyao, A., Kojima, M., Sakakibara, H., Mizutani, M., Morita, H., Toda, Y., Hobo, T., Sato, Y., Hattori, T., Hirohiko, H. and Takeda S.
  • Organizer: 10th International Congress on Plant Molecular Biology
  • Place of Presentation: Jeju, Korea
• [Presentation] RSS3 forms a complex with JAZ and bHLH factors, and controls adaptive root growth under salinity stress in rice. (2012)
  • Author(s): Toda, Y. Tanaka, M. Ogawa, D. et.al.
  • Organizer: 10th International Congress on Plant Molecular Biology
  • Place of Presentation: Jeju, Korea
• [Patent(Industrial Property Rights)] 環境ストレス耐性を付与する遺伝子及びその利用 (2014)
  • Inventor(s): 武田真、服部束穂、黒谷賢一、市川裕章
  • Industrial Property Rights Holder: 名古屋大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-11-19