| Project/Area Number |
24580494
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Okayama University |
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Principal Investigator |
MAEDA Megumi 岡山大学, 環境生命科学研究科, 助教 (20434988)
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| Co-Investigator(Renkei-kenkyūsha) |
KIMURA Yoshinobu 岡山大学, 大学院環境生命科学研究科, 教授 (70195384)
OTSUKI Takemi 川崎医科大学, 衛生学, 教授 (40160551)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | N-グリカン / 植物抗原性糖鎖 / スギ花粉アレルゲン / Th2免疫応答抑制 / 多量精製 / ルイスa抗原 / 糖鎖ポリマー / 樹状細胞 / Cry j1 / Asn-糖鎖 / γポリグルタミン酸 / サイトカイン / 淡水性植物 / T細胞 / Th2応答 / 植物抗原性 / 親水性クロマト / γ-ポリグルタミン酸 / ヒト樹状細胞 |
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| Outline of Final Research Achievements |
The plant antigenic N-glycans from plant glycoproteins have suppressed Th2-type immune responses by Japanese cedar pollen allergen, suggesting that the plant antigenic N-glycans could become effective agents in therapy for patients with pollinosis. In this study, a new method with hydrophilic partitioning was developed to prepare the large-scale of Asn-glycopeptide bearing plant antigenic N-glycans from Ginkgo seeds. And then, plant antigenic N-glycans bearing Lewis a antigen(s) at the non-reducing terminal were found in submerged plants. Finally, the glycol-polymer bearing multivalent plant antigenic N-glycans was synthesized, and the differential inhibition of dendritic cells by the glycol-polymer was analyzed.
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