Functional analysis of cytosolic free N-glycans involved in regulation of plant growth and application of the biofunction of free N-glycans
| Project/Area Number |
24580495
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Okayama University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 植物糖タンパク質 / 植物糖鎖 / 遊離 N-グリカン / PNGase / ENGase / トランスジェニック植物 / ペプチド: N-グリカナーゼ (PNGase) / α-フコシダーゼ (α-Fuc’ase) / 植物N-グリカン / N-グリカン代謝 / 遺伝子ノックアウト植物 / 遊離N-グリカン / 糖鎖機能 / エンドグリコシダーゼ (ENGase) / 遺伝子発現抑制変異株 / 遺伝子過剰発現変異株 |
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| Outline of Final Research Achievements |
In developing plant tissues, free N-glycan that are enzymatically liberated from glycoproteins occur ubiquitously at micromolar level. As a part of study to elucidate the physiological function(s) of these free N-glycans involved in plant development, we constructed knock-out or knock-down plants, in which the expressions of ENGase or PNGase genes were suppressed. The structural features of free N-glycans expressed in those transgenic plants were clearly distinguishable from those expressed in wild type plants. Although significant difference in phenotype between the transgenic plants and wild type was not observed, a specific difference in the fruit-ripening process was observed.
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Report
(4 results)
Research Products
(58 results)
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[Journal Article] Purification and Molecular Characterization of Ginkgo biloba β-Xylosidase Active for Plant Complex Type N-Glycans.2013
Author(s)
Maeda, M., Akiyama, T., Yokouchi, D., Woo, K. K., and Kimura, Y.
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Journal Title
Biosci. Biotechnol. Biochem.,
Volume: 77
Pages: 1973-1976
Related Report
Peer Reviewed
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[Journal Article] Large-Scale Preparation of Asn-Glycopeptide Carrying Structurally Homologous Antigenic N-Glycan2013
Author(s)
Maeda, M., Takeda, N., Mano, A., Yamanishi, M., Kimura, M., and Kimura, Y.
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Journal Title
Biosci. Biotechnol. Biochem.,
Volume: 77
Pages: 1269-1274
NAID
Related Report
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[Journal Article] Alteration of cytoskeletal molecules in a human T cell line caused by continuous exposure to chrysotile asbestos.2013
Author(s)
Kumagai-Takei, N., Hayashi, H., Matzuzaki, H., Lee, S., Hirastauka, J-i., Nishimura, Y., Kimura, Y., Ostuki, T
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Journal Title
Immunobiology.
Volume: 218
Pages: 1184-1191
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[Presentation] PREPARATION AND IMMUNOLOGICAL ACTIVITY OF NEOGLYCOCONJUGATE WITH PLANT ANTIGENIC N-GLYCANS2012
Author(s)
Maeda, M., Mano, A.2; Yamanishi, M., Otsuki, T., Okano, M., Kimura, Y.
Organizer
26th International Carbohydrate Symposium
Place of Presentation
Madrid, Spain
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