| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
We performed the nuclear magnetic resonance analysis of the sialic acid-binding mechanism of the novel sialic acid-binding lectin (SRC) produced from the C-terminal domain of the novel R-type lectin from the earthworm (EW29Ch) by natural evolution-mimicry. The results showed that in the 6’-sialyllactose-bound state SRC has two conformations different from both the conformation in the sugar-free state and that in the lactose-bound state. Further, the internal motion of the loop region in subdomain γ of SRC remarkably changed from that of the corresponding region in subdomain γ of the wild type lectin EW29Ch, indicating that the internal motion change is important in the sialic acid-binding mechanism of SRC.
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