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Analysis of role of fructan exohydrolase genes in efficient use of wintering energy of wheat

Research Project

Project/Area Number 24580501
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

YOSHIDA Midori  独立行政法人農業・食品産業技術総合研究機構, 北海道農業研究センター・寒地作物研究領域, 主任研究員 (00355455)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsフルクタン / 糖代謝 / コムギ / 耐凍性 / 雪腐病抵抗性 / 越冬性 / 耐雪性
Outline of Final Research Achievements

In autumn, winter wheat accumulates fructan, which is a kind of fructose-based oligo- and polysaccharide with high hydrophilicity, and the content of fructan in wheat is associated with both freezing tolerance and snow mold resistance. Recently, we cloned several kinds of genes encoding FEH (fructan exohydrolase) from wheat, and analyses of their substrate specificities revealed that various FEHs, including 1-FEH, 6-FEH, 6-KEH (kestose exohydrolase) and 6&1-FEH, exist in wheat plants. Analysis of gene expression of these FEHs during a wintering period showed that seasonal changes in mRNA levels were different among genes. Seasonal changes and varietal difference in expressions of FEH genes might also play an important role in tolerance to environmental stresses during wintering.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (6 results)

All 2014 2013 2012 Other

All Presentation (6 results) (of which Invited: 1 results)

  • [Presentation] Fructan metabolism associated with freezing tolerance and snow mold resistance of winter wheat in Hokkaido2014

    • Author(s)
      Midori Yoshida
    • Organizer
      NARO/HARC International Seminar on Molecular Biology for Stress Tolerances in Rice and Upland Crops
    • Place of Presentation
      札幌 かでる27
    • Year and Date
      2014-10-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] コムギの越冬能力に関わるフルクタン分解酵素遺伝子群の発現解析2013

    • Author(s)
      吉田みどり, 目黒文乃, 川上顕
    • Organizer
      第54回日本植物生理学会
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Research-status Report
  • [Presentation] Molecular Analysis of fructan metabolism associated with freezing tolerance and snow mold resistance of winter wheat2012

    • Author(s)
      Midori Yoshida, Akira Kawakami
    • Organizer
      Plant and Microbe Adaptation to Cold 2012
    • Place of Presentation
      北海道大学学術交流会館
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Fructan exohydrolase involved in graminan breakdown in winter wheat infected by snow mold under snow cover2012

    • Author(s)
      Akira Kawakam, Midori Yoshida
    • Organizer
      Plant and Microbe Adaptation to Cold 2012
    • Place of Presentation
      北海道大学学術交流会館
    • Related Report
      2012 Research-status Report
  • [Presentation] Expression analysis of fructan exohydrolase genes in winter wheat2012

    • Author(s)
      Midori Yoshida, Akira Kawakami
    • Organizer
      7th International Fructan Symposium
    • Place of Presentation
      フランス、サンジャンルトマ
    • Related Report
      2012 Research-status Report
  • [Presentation] 秋播きコムギの雪腐病抵抗性に関与するフルクタン分解酵素遺伝子

    • Author(s)
      吉田みどり、目黒文乃、川上顕
    • Organizer
      日本植物学会 第77回大会研究発表記録
    • Place of Presentation
      北海道大学
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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