Development of a method to screen MS-silent post-translational modifications
| Project/Area Number |
24590044
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Tohoku University |
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Principal Investigator |
GOTO Takaaki 東北大学, 薬学研究科(研究院), 講師 (40344684)
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| Co-Investigator(Kenkyū-buntansha) |
OE Tomoyuki 東北大学, 大学院薬学研究科, 教授 (10203712)
LEE Seon Hwa 東北大学, 大学院薬学研究科, 助教 (60519776)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 分析科学 / 生体分子 / タンパク質 / 質量分析 / 翻訳後修飾 / プロテオーム |
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| Outline of Final Research Achievements |
A novel methodology to screen MS-silent post-translational modifications on protein has been examined. This method is based on MS-based proteomics combined with the group-specific immunoaffinity extraction, complementary data analyses in both positive and negative mode, and LC/high-resolution MS. This strategy can be used for screening of modifications including isomerization.
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Report
(4 results)
Research Products
(19 results)
