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Development of a method to screen MS-silent post-translational modifications

Research Project

Project/Area Number 24590044
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Physical pharmacy
Research InstitutionTohoku University

Principal Investigator

GOTO Takaaki  東北大学, 薬学研究科(研究院), 講師 (40344684)

Co-Investigator(Kenkyū-buntansha) OE Tomoyuki  東北大学, 大学院薬学研究科, 教授 (10203712)
LEE Seon Hwa  東北大学, 大学院薬学研究科, 助教 (60519776)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords分析科学 / 生体分子 / タンパク質 / 質量分析 / 翻訳後修飾 / プロテオーム
Outline of Final Research Achievements

A novel methodology to screen MS-silent post-translational modifications on protein has been examined. This method is based on MS-based proteomics combined with the group-specific immunoaffinity extraction, complementary data analyses in both positive and negative mode, and LC/high-resolution MS. This strategy can be used for screening of modifications including isomerization.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (19 results)

All 2014 2013 2012

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 1 results) Presentation (14 results) (of which Invited: 1 results)

  • [Journal Article] Mass spectrometric characterizations of protein carbonylation: Comparison between three different conditions, oxidation by CuII/ascorbic acid, adduction of methyl glyoxal, and adduction of 4-hydroxy-2(E)-nonenal2014

    • Author(s)
      Kazuki Maeda, Hiroyuki Funatsu, Yuki Miyazawa, Yasushi Misawa, Takaaki Goto, Seon Hwa Lee and Tomoyuki Oe
    • Journal Title

      International Journal of Mass Spectrometry

      Volume: 373 Pages: 72-80

    • DOI

      10.1016/j.ijms.2014.09.012

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Predicted multiple selected reaction monitoring to screen activated drug-mediated modifications on human serum albumin.2014

    • Author(s)
      Fumio Osaki, Takaaki Goto, Seon Hwa Lee and Tomoyuki Oe
    • Journal Title

      Analytical Biochemistry

      Volume: 449 Pages: 59-67

    • DOI

      10.1016/j.ab.2013.12.016

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Complete amino acid sequencing and immunoaffinity clean-up can facilitate screening of various chemical modifications on human serum albumin.2013

    • Author(s)
      Takaaki Goto, Kazuyuki Murata, Seon Hwa Lee and Tomoyuki Oe
    • Journal Title

      Analytical and Bioanalytical Chemistry

      Volume: 405 Issue: 23 Pages: 7383-7395

    • DOI

      10.1007/s00216-013-7146-0

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Aldehyde stress-mediated novel modification of proteins: epimerization of the N-terminal amino acid.2013

    • Author(s)
      Ryo Kajita, Takaaki Goto, Seon Hwa Lee and Tomoyuki Oe
    • Journal Title

      Chemical Research in Toxicology

      Volume: 26 Issue: 12 Pages: 1926-1936

    • DOI

      10.1021/tx400354d

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Can Edman degradation be used for quantification? Isotope-dilution liquid chromatography-electrospray ionization tandem mass spectrometry and the long-term stability of 20 phenylthiohydantoin-amino acids2013

    • Author(s)
      Satoh, R., Goto, T., Lee, S.H., Oe, T.
    • Journal Title

      Anal. Bioanal. Chem

      Volume: (in press) Issue: 25 Pages: 8001-8010

    • DOI

      10.1007/s00216-013-6901-6

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 血清アルブミン上の網羅的化学修飾解析を指向したLC/MSによる手法の開発2014

    • Author(s)
      工藤裕太、李宣和、後藤貴章、大江知行
    • Organizer
      第53回日本薬学会東北支部大会
    • Place of Presentation
      いわき
    • Year and Date
      2014-10-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] 血清アルブミンの化学修飾オミクス研究:新規バイオマーカー開発に向けて2014

    • Author(s)
      後藤貴章
    • Organizer
      平成26年度日本薬学会東北支部 第3回 物理・分析系若手研究者セミナー
    • Place of Presentation
      仙台
    • Year and Date
      2014-09-20
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Simultaneous mass spectrometric analysis of various chemical modifications on human serum albumin: strategies for clean-up, sequence coverage, and identification2014

    • Author(s)
      Takaaki Goto, Yuta Kudo, Kazuyuki Murata, Seon Hwa Lee and Tomoyuki Oe
    • Organizer
      62nd ASMS Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      Baltimore, USA
    • Year and Date
      2014-06-15 – 2014-06-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] 血清アルブミン上の網羅的化学修飾解析を目的とした高分解能LC/MSによる修飾ペプチド検出法の検討2014

    • Author(s)
      後藤貴章、工藤裕太、李宣和、大江知行
    • Organizer
      第74回分析化学討論会
    • Place of Presentation
      郡山
    • Year and Date
      2014-05-24 – 2014-05-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] シッフ塩基形成を介した血清アルブミンN末端アミノ酸の光学反転2013

    • Author(s)
      梶田遼、後藤貴章、李宣和、大江知行
    • Organizer
      平成25年度日本分析化学会東北支部若手交流会
    • Place of Presentation
      仙台
    • Related Report
      2013 Research-status Report
  • [Presentation] 標品不要のタンパク質・ペプチド絶対定量法の開発: エドマン分解反応物の同位体希釈LC-ESI/MS/MS法2013

    • Author(s)
      佐藤涼、後藤貴章、李宣和、大江知行
    • Organizer
      平成25年度日本分析化学会東北支部若手交流会
    • Place of Presentation
      仙台
    • Related Report
      2013 Research-status Report
  • [Presentation] アルデヒドによるN末端アミノ酸の異性化における反応性の検討2013

    • Author(s)
      東由佳、後藤貴章、李宣和、大江知行
    • Organizer
      第52回日本薬学会東北支部大会
    • Place of Presentation
      仙台
    • Related Report
      2013 Research-status Report
  • [Presentation] Predicted Selected Reaction Monitoring を用いたヘモグロビン上の化学修飾スクリーニング法の開発2013

    • Author(s)
      小島弘貴、李宣和、後藤貴章、大江知行
    • Organizer
      第52回日本薬学会東北支部大会
    • Place of Presentation
      仙台
    • Related Report
      2013 Research-status Report
  • [Presentation] アルデヒドによるタンパク質N末端アミノ酸異性化の研究2013

    • Author(s)
      梶田遼、李宣和、後藤貴章、大江知行
    • Organizer
      新アミノ酸分析研究会 第3回学術講演会
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Presentation] Mechanistic Study on Non-enzymatic Isomerization of N-terminal Amino Acids by Endogenous Aldehydes2012

    • Author(s)
      Ryo Kajita, Takaaki Goto, Seon Hwa Lee and Tomoyuki Oe
    • Organizer
      19th International Mass Spectrometry Conferrence
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2012 Research-status Report
  • [Presentation] Isotope dilution LC-ESI/MS/MS method for the determination of phenylthiohydantoin (PTH)-amino acids derived from Edman degradation products of proteins2012

    • Author(s)
      Ryo Satoh, Takaaki Goto, Seon Hwa Lee and Tomoyuki Oe
    • Organizer
      19th International Mass Spectrometry Conferrence
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2012 Research-status Report
  • [Presentation] Chemical modificomics ~Alternative analytical platform for efficient biomarker discovery~2012

    • Author(s)
      Tomoyuki Oe, Takaaki Goto, Shota Kojima, Shohei Shitamichi, Kazuyuki Murata, Kohei Miyamoto and Seon Hwa Lee
    • Organizer
      19th International Mass Spectrometry Conferrence
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2012 Research-status Report
  • [Presentation] N末端アミノ酸のpyridoxal 5'-phosphateに対する反応性と非酵素的異性化2012

    • Author(s)
      梶田遼、東由佳、後藤貴章、李宣和、大江知行
    • Organizer
      第25回バイオメディカル分析科学シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] タンパク質のエドマン分解反応物: PTH-アミノ酸を用いた同位体希釈LC/MS法の構築2012

    • Author(s)
      佐藤涼、後藤貴章、李宣和、大江知行
    • Organizer
      新アミノ酸分析研究会 第二回学術講演会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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