Detailed study on the immunoactive sequence of glycosaminoglycans.

• Project/Area Number: 24590046
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Chiba University
• Principal Investigator: TOIDA Toshihiko 千葉大学, 薬学研究科(研究院), 教授 (60163945)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
• Keywords: グリコサミノグリカン / 免疫賦活活性 / 糖鎖配列 / コンドロイチン硫酸 / 活性中心 / 経口吸収 / 質量分析 / 二酸化チタン / 光分解 / 低分子化

Outline of Final Research Achievements

We have isolated and characterized new chondroitin sulfate sampls from several shark cartilage and examined their binding property to some important biological substances such as midkine and preiotrophin. Furthermore, we have looked at in vivo effects of new chondroitin sulfates on elongation of neurites of mouse embryonic nuroblasts and have found that new chondroitin sulfate samples showed strong effects on neurite elongation compared to usual chondroitin sulfate isolated from whale/bovine cartilage.

Research Products

• [Journal Article] Polyphenol Extract from Phellinus igniarius Protects against Acrolein Toxicity In Vitro and Provides Protection in a Mouse Stroke Model. (2015)
  • Author(s): Suabjakyong P, Saiki R, Van Griensven LJ, Higashi K, Nishimura K, Igarashi K, Toida T.
  • Journal Title: Plos One Volume: 10 Issue: 3 Pages: e0122733-e0122733
  • DOI: 10.1371/journal.pone.0122733
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Composition of Glycosaminoglycans in Elasmobranchs including Several Deep-Sea Sharks: Identification of Chondroitin/Dermatan Sulfate from the Dried Fins of Isurus oxyrinchus and Prionace glauca. (2015)
  • Author(s): Higashi K, Takeuchi Y, Mukuno A, Tomitori H, Miya M, Linhardt RJ, Toida T.
  • Journal Title: Plos One Volume: 10 Issue: 3 Pages: e0120860-e0120860
  • DOI: 10.1371/journal.pone.0120860
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Distinguishing mild cognitive impairment from Alzheimer's disease with acrolein metabolites and creatinine in urine. (2015)
  • Author(s): Yoshida M, Higashi K, Kuni K, Mizoi M, Saiki R, Nakamura M, Waragai M, Uemura K, Toida T, Kashiwagi K, Igarashi K.
  • Journal Title: Clin Chim Acta Volume: 441 Pages: 115-121
  • Peer Reviewed / Open Access
• [Journal Article] Identification of functional amino acid residues involved in polyamine and agmatine transport by human organic cation transporter 2. (2014)
  • Author(s): Higashi, K. et al.
  • Journal Title: PLoS One Volume: 9 Issue: 7 Pages: e102234-e102234
  • DOI: 10.1371/journal.pone.0102234
  • Peer Reviewed / Open Access
• [Journal Article] Method to detect contaminants in heparin using radical depolymerization and liquid chromatography-mass spectrometry. (2014)
  • Author(s): Li G, Cai C, Li L, Fu L, Chang Y, Zhang F, Toida T, Xue C, Linhardt RJ.
  • Journal Title: Analytical Chemistry Volume: 86 Issue: 1 Pages: 326-330
  • DOI: 10.1021/ac403625a
  • Peer Reviewed / Open Access
• [Journal Article] Properties of putrescine uptake by PotFGHI and PuuP and their physiological significance in Escherichia coli. (2014)
  • Author(s): Terui, Y. et al.
  • Journal Title: Amino Acids Volume: 46 Issue: 3 Pages: 661-670
  • DOI: 10.1007/s00726-013-1517-x
  • Peer Reviewed
• [Journal Article] Acrolein stimulates the synthesis of IL-6 and CRP in thrombosis model mice and cultured cells. (2013)
  • Author(s): Saiki, R. et al.
  • Journal Title: J. Neurochem. Volume: 127 Issue: 5 Pages: 652-659
  • DOI: 10.1111/jnc.12336
  • Peer Reviewed
• [Journal Article] Acetaldehyde-induced cytotoxicity involves induction of spermine oxidase at the transcriptional level. (2013)
  • Author(s): Uemura, T. et al.
  • Journal Title: Toxicology Volume: 310 Pages: 1-7
  • DOI: 10.1016/j.tox.2013.05.008
  • Peer Reviewed
• [Journal Article] In vitro and in vivo evaluation of polymethylene tetraamine derivatives as NMDA receptor channel blockers. (2013)
  • Author(s): Saiki, R. et al.
  • Journal Title: Bioorg. Med. Chem. Lett. Volume: 23 Issue: 13 Pages: 3901-3904
  • DOI: 10.1016/j.bmcl.2013.04.063
  • Peer Reviewed
• [Journal Article] Sequence analysis and domain motifs in the porcine skin decorin glycosaminoglycan chain. (2013)
  • Author(s): Zhao X, Yang B, Solakylidirim K, Joo EJ, Toida T, Higashi K, Linhardt RJ, Li L.
  • Journal Title: J Biol Chem. Volume: 288 Issue: 13 Pages: 9226-9237
  • DOI: 10.1074/jbc.m112.437236
  • Peer Reviewed
• [Journal Article] Simultaneous determination of uronates found in polysaccharides from natural products by HPLC with fluorometric detection. (2012)
  • Author(s): Matsumoto A, Hosoyama S, Higashi K, Toida T.
  • Journal Title: Carbohydr Res. Volume: 358 Pages: 82-88
  • DOI: 10.1016/j.carres.2012.06.018
  • Peer Reviewed
• [Journal Article] Structural change of R domain heterodimer of NMDA receptor GluN1 and GluN2B through binding of spermine and ifenprodil. (2012)
  • Author(s): Tomitori, H.
  • Journal Title: J. Pharmacol. Exp. Ther. Volume: 343 Issue: 1 Pages: 82-90
  • DOI: 10.1124/jpet.112.192286
  • Peer Reviewed
• [Journal Article] Inverse correlation between stroke and urinary 3-hydroxypropyl mercapturic acid, an acrolein- glutathione metabolite (2012)
  • Author(s): Yoshida, M
  • Journal Title: Clin. Chim. Acta Volume: 413(7-8) Issue: 7-8 Pages: 753-9
  • DOI: 10.1016/j.cca.2012.01.020
  • Peer Reviewed
• [Presentation] Effect of chondroitin sulfate on intestinal epitherial cells. (2014)
  • Author(s): Toida T
  • Organizer: Glycomics
  • Place of Presentation: Troy, RPI USA
  • Year and Date: 2014-07-20
  • Invited
• [Presentation] Study of chondroitin sulfate on immunosysytem. (2014)
  • Author(s): Toida T
  • Organizer: Gordon Research Conference
  • Place of Presentation: Proctor Academy, USA
  • Year and Date: 2014-07-15 – 2014-07-20
• [Presentation] シロキクラゲ由来多糖類の構造と生理活性 (2014)
  • Author(s): 戸井田敏彦
  • Organizer: 日本薬学会年会
  • Place of Presentation: 熊本
• [Presentation] センチネルリンパ節検出用多糖類の検索 (2013)
  • Author(s): 戸井田敏彦
  • Organizer: 日本糖質学会年会
  • Place of Presentation: 大阪
• [Presentation] Regulation of heparin injections (2013)
  • Author(s): Toshihiko Toida
  • Organizer: American Chemical Society
  • Place of Presentation: New Orleans, LA, USA
  • Invited
• [Presentation] マフノリ由来硫酸化多糖の構造と免疫賦活活性 (2013)
  • Author(s): 東恭平、戸井田敏彦
  • Organizer: 第１３２回日本薬学会
  • Place of Presentation: 横浜
• [Presentation] New over sulfonated chondroitin sulfate (2012)
  • Author(s): Toshihiko Toida, Kyouhei Higashi
  • Organizer: Gordon Research Conference "Proteoglycans"
  • Place of Presentation: Andover, NH, USA
• [Presentation] 深海鮫由来コンドロイチン硫酸の構造 (2012)
  • Author(s): 東恭平、戸井田敏彦
  • Organizer: 日本糖質学会
  • Place of Presentation: 鹿児島
• [Remarks] 千葉大学大学院薬学研究院病態分析化学研究室ホームページ
  • URL: http://www.p.chiba-u.jp/lab/bunseki/index.html
• [Remarks] 千葉大学大学院薬学研究院病態分析化学研究室ホームページ
  • URL: http://www.p.chiba-u.ac.jp/lab/bunseki/index.html
• [Remarks] 千葉大学大学院薬学研究院病態分析化学研究室ホームページ
  • URL: http://www.p.chiba-u.ac.jp/lab/bunseki/index.html