Verification of the connector function of the transcription and splicing factor PQBP1

• Project/Area Number: 24590049
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: University of Toyama
• Principal Investigator: MIZUGUCHI Mineyuki 富山大学, 医学薬学研究部(薬学), 教授 (30332662)
• Co-Investigator(Renkei-kenkyūsha): OBITA Takayuki 富山大学, 大学院医学薬学研究部(薬学), 准教授 (30578696)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: タンパク質 / 天然変性蛋白質 / 解離定数 / 表面プラズモン共鳴 / 天然変性タンパク質

Outline of Final Research Achievements

The transcription and splicing factor PQBP1 is composed of a small folded WW domain and a long disordered region. In the present study, we quantitatively analyzed the interaction of PQBP1 with spliceosomal proteins WBP11 and U5-15kD using surface plasmon resonance technique. In addition, we also demonstrated that PQBP1 simultaneously binds to WBP11 and U5-15kD. Our results suggest that PQBP1 acts as a connector protein in spliceosome.

Research Products

• [Journal Article] Segmental isotope-labeling of the intrinsically disordered protein PQBP1. (2014)
  • Author(s): Nabeshima Y, Mizuguchi M, Kajiyama A, Okazawa H.
  • Journal Title: FEBS Lett. Volume: 588 Issue: 24 Pages: 4583-9
  • DOI: 10.1016/j.febslet.2014.10.028
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Mutations in the gene PQBP1 prevent its interaction with the spliceosomal protein U5-15kD (2014)
  • Author(s): Mizuguchi, M., Obita, T., Serita, T., Kojima, R., Nabeshima, Y., and Okazawa, H.
  • Journal Title: Nat Commun. Volume: 5 Issue: 1 Pages: 3822-3822
  • DOI: 10.1038/ncomms4822
  • Peer Reviewed
• [Journal Article] Structural Study of Polyglutamine Tract-binding Protein 1 (2013)
  • Author(s): Mizuguchi, M., Okazawa, H.
  • Journal Title: YAKUGAKU ZASSHI Volume: 133 Issue: 5 Pages: 519-526
  • DOI: 10.1248/yakushi.13-00001-2
  • NAID: 130003361945
  • ISSN: 0031-6903, 1347-5231
  • Year and Date: 2013-05-01
  • Peer Reviewed
• [Presentation] 天然変性蛋白質PQBP1のセグメント同位体標識 (2014)
  • Author(s): 9) 鍋島裕子，水口峰之，梶山亜沙希，岡澤均
  • Organizer: 日本薬学会北陸支部第126回例会
  • Place of Presentation: 金沢市
  • Year and Date: 2014-11-16
• [Presentation] セグメントラベル法によるタンパク質の重水素化 (2014)
  • Author(s): 水口峰之
  • Organizer: 平成25年度第2回生物構造学研究会
  • Place of Presentation: 東京
  • Invited
• [Presentation] 天然変性蛋白質PQBP1のセグメント同位体標識 (2013)
  • Author(s): 水口峰之，鍋島裕子
  • Organizer: 第52回NMR討論会
  • Place of Presentation: 金沢
• [Presentation] 天然変性蛋白質PQBP1のセグメント標識 (2012)
  • Author(s): 水口峰之，鍋島裕子
  • Organizer: 第51回NMR討論会
  • Place of Presentation: 名古屋市
• [Book] 13章天然変性タンパク質．「揺らぎ・ダイナミクスと生体機能 物理化学的視点から見た生体分子，寺嶋正秀 編」 (2013)
  • Author(s): 水口峰之，片岡幹雄
  • Total Pages: 8
  • Publisher: 化学同人
• [Remarks] 構造生物学研究室の研究成果が Nature Communicationsに掲載されました
  • URL: http://www.pha.u-toyama.ac.jp/news/2014.05.01.html
• [Remarks] PQBP1遺伝子変異が関与する知的障害の原因を解明（プレスリリース）
  • URL: http://www.u-toyama.ac.jp/outline/publicity/pdf/2014/5.pdf