| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
On the basis of the principal of our original phosphate affinity electrophoresis technology (Phos-tag SDS-PAGE) , we have developed a system that permits to separate various cellular proteins with a wide range of molecular masses. A Bis-Tris-buffered gel was optimum for the analysis of phosphoproteins with molecular masses of less than 200kDa. For analyzing high-molecular-mass proteins with more than 200 kDa, on the other hand, a Tris-AcOH-buffered gel was optimum. We are now investigating phosphorylation states of a wide variety of cellular proteins using this system and are in the process of accumulating data to be open to the public on our Web site.
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