Development of phosphate affinity electrophoresis system for phosphorylation profiling of proteins with a wide range of molecular masses.
Project/Area Number |
24590050
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Physical pharmacy
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Research Institution | Hiroshima University |
Principal Investigator |
KINOSHITA Emiko 広島大学, 医歯薬保健学研究院, 助教 (40379912)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | リン酸基親和性電気泳動 / Phos-tag / フォスタグ / リン酸基親和性電気泳動法 / 亜鉛フォスタグ / タンパク質 / 分析化学 / リン酸化シグナル |
Outline of Final Research Achievements |
On the basis of the principal of our original phosphate affinity electrophoresis technology (Phos-tag SDS-PAGE) , we have developed a system that permits to separate various cellular proteins with a wide range of molecular masses. A Bis-Tris-buffered gel was optimum for the analysis of phosphoproteins with molecular masses of less than 200kDa. For analyzing high-molecular-mass proteins with more than 200 kDa, on the other hand, a Tris-AcOH-buffered gel was optimum. We are now investigating phosphorylation states of a wide variety of cellular proteins using this system and are in the process of accumulating data to be open to the public on our Web site.
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Report
(4 results)
Research Products
(41 results)
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[Journal Article] Simple enrichment of thiol- containing biomolecules by using zinc(II)-cyclen-functionalized magnetic beads2014
Author(s)
Fujioka, H., Tsunehiro, M., Kawaguchi, M., Kuramoto, Y., Kurosaki, H., Hieda, Y., Kinoshita-Kikuta, E., Eiji Kinoshita, and Koike, T.
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Journal Title
J. Sep. Sci.
Volume: 37
Issue: 13
Pages: 1601-1609
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Increase in Lipophilicity of Enalaprilat by Complexation with Copper(II) or Zinc(II) Ions.2013
Author(s)
Fujioka, H., Hieda, Y., Kuramoto, Y, Konishi, K., Kinoshita-Kikuta, E., Eiji Kinoshita, and Koike T.
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Journal Title
Yakugaku Zasshi
Volume: 33
Pages: 1135-1141
Related Report
Peer Reviewed
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