Synthesis of interigent derivatization reagents and discovery of disease-related biomarkers based on chiral metabolome analysis
| Project/Area Number |
24590055
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | University of Shizuoka |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 光学活性誘導体化試薬 / キラルメタボロミクス / バイオマーカー探索 / 疾病診断 / 液体クロマトグラフィー / 質量分析 / 質量分析法 / 光学活性アミノ酸 / 光学活性カルボン酸 / 光学活性アミン |
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| Outline of Final Research Achievements |
This study deals with the synthesis of new chiral derivatization reagents for the enantiomers of amines and carboxyls in LC-MS/MS analysis and their application to chiral metobolomics.
(S)-PyT-N, PCP2 and DMT-3(S)-Apy were designed and synthesized as the reagents for chiral carboxyls, whereas (R)-PyT-C, DMT-L-Pro-OSu, L-PGA and L-PGA-OSu were developed for chiral amines. L-PGA-d5, an isotopic valiant of L-PGA, was also synthesized to avoid the matrics effect in LC-MS analysis. The precise determination of D/L-amino acids was performed using the light and heavy reagents. Furthermore, DMT-3(S)-Apy was applied for the determination of carboxyls in saliva of diabetic patients. The ratio of D/L-lactic acids in the saliva of diabetic patients was higher than that of healthy persons. This result suggests that the determination of chiral metabolites is efficient for biomarker discovery related for some diseases.
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Report
(4 results)
Research Products
(33 results)
