| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
This study deals with the synthesis of new chiral derivatization reagents for the enantiomers of amines and carboxyls in LC-MS/MS analysis and their application to chiral metobolomics.
(S)-PyT-N, PCP2 and DMT-3(S)-Apy were designed and synthesized as the reagents for chiral carboxyls, whereas (R)-PyT-C, DMT-L-Pro-OSu, L-PGA and L-PGA-OSu were developed for chiral amines. L-PGA-d5, an isotopic valiant of L-PGA, was also synthesized to avoid the matrics effect in LC-MS analysis. The precise determination of D/L-amino acids was performed using the light and heavy reagents. Furthermore, DMT-3(S)-Apy was applied for the determination of carboxyls in saliva of diabetic patients. The ratio of D/L-lactic acids in the saliva of diabetic patients was higher than that of healthy persons. This result suggests that the determination of chiral metabolites is efficient for biomarker discovery related for some diseases.
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