| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
In order to facilitate drug discovery for cancer, we aim to elucidate the activating mechanism of the FGFR3 pathogenic mutants by the functional and crystallographic analyses. The Km (ATP) values of N540T, K650M, and G697C mutants varied from 0.30- to 108-hold compared with that of the wild type. The result implies that these mutants are activated via distinguished mechanisms. The exploration of crystallization condition has yielded the micro-crystals of the wild type and mutants. Ongoingly, we will perform the crystal analyses and elucidate the molecular mechanisms for the activation of the active mutants.
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