| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
In this study, as part of development of technique for higher order structure of glycoprotein, structural analysis of the anti-TNF-α agents/human soluble TNF-α interaction was performed by hydrogen deuterium exchange/mass spectrometry (HDX/MS). HDX/MS of the TNF-α with or without anti-TNF-α agents was revealed that the 4 agents protected common internal regions of TNF-α. This result suggested that anti-TNF-α agents exhibit pharmaceutical effects via common structural changes of TNF-α. Additionally, characteristic epitopes of these anti-TNF-α agents were also identified by HDX/MS analysis.
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