| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The aim of this study was to characterize the amino acid residues responsible for the substrate specificity as well as to elucidate the biological functions of hyaluronidase 4 (HYAL4), which is a chondroitin sulfate-specific hydrolase. A series of HYAL4/other hyaluronidases-chimeric proteins and HYAL4 point mutants have been generated, and their preference for substrates has been investigated. The amino acid residues required for the substrate specificity of HYAL4 have been identified. The expression patterns as well as periods of HYAL4 mRNA have also been analyzed in detail. The relation between some diseases and HYAL4 has been investigated, and high expression of HYAL4 in some metastatic cancer cell lines has been demonstrated. We have also succeeded in the preparation of the targeting vector for generation of Hyal4-deficient mice.
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