Analysis of the mechanism of the regulation of proteasome by novel modifier PI31

• Project/Area Number: 24590075
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: The University of Tokyo
• Principal Investigator: HAMAZAKI Jun 東京大学, 薬学研究科(研究院), 助教 (80533588)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: プロテアソーム / タンパク質分解 / ユビキチン

Outline of Final Research Achievements

Proteasome is a essential protease complex that is conserved in eukaryotes. Recently, we identified PI31 as a novel proteasome function modifier. We observed PI31 knockdown fly shows signify defect in development and cell homeostasis. We conducted the biochemical study about PI31 and generated the PI31 conditional knockout mice.

Research Products

• [Journal Article] Characterization of Testis-Specific Proteasome Subunit α4s in Mammals (2014)
  • Author(s): Hiroyuki Uechi, Jun Hamazaki, and Shigeo Murata
  • Journal Title: The Journal of Biological Chemistry Volume: 289 Issue: 18 Pages: 12365-12374
  • DOI: 10.1074/jbc.m114.558866
  • Peer Reviewed
• [Journal Article] Mouse zygote-specific proteasome assembly chaperone important for maternal-to-zygotic transition (2013)
  • Author(s): Shin SW
  • Journal Title: Biolology Open Volume: 2 Issue: 2 Pages: 170-182
  • DOI: 10.1242/bio.20123020
  • Peer Reviewed
• [Presentation] プロテアソーム相互作用因子PI31の機能解析 (2014)
  • Author(s): 治田義太郎、濱崎純、八代田英樹、村田茂穂
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 哺乳類プロテアソームサブユニットRpn10とRpn13による協調したユビキチン認識はプロテアソーム機能に重要である (2014)
  • Author(s): 濱崎純、村田茂穂
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] A heat shock proteins- and ubiquitin-interacting protein counteracts aggregate formation of ubiquitinated proteins for proteasome degradation (2014)
  • Author(s): Hiroyuki Uechi, Jun Hamazaki, Erina Kuranaga, Masayuki Miura, and Shigeo Murata
  • Organizer: Symposium for young ubiquitin researchers in Japan "New Era in the Ubiquitin Research"
  • Place of Presentation: 国際高等研究所 (京都)
  • Year and Date: 2014-11-11 – 2014-11-12
• [Presentation] 哺乳類プロテアソームにおけるユビキチン化タンパク質認識機構と脱ユビキチン化機構の遺伝学的解析 (2014)
  • Author(s): 濱崎純、村田茂穂
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 国立京都国際会館・グランドプリンスホテル京都
  • Year and Date: 2014-10-15 – 2014-10-18
  • Invited
• [Presentation] A novel ubiquitin-interacting protein that counteracts aggregate formation of ubiquitinated proteins (2014)
  • Author(s): Hiroyuki Uechi, Jun Hamazaki, Erina Kuranaga, Masayuki Miura, and Shigeo Murata
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 国立京都国際会館・グランドプリンスホテル京都 (京都)
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] Collaborative roles of proteasome ubiquitin receptors Rpn13 and Rpn10 are essential for hepatocyte homeostasis (2014)
  • Author(s): Jun Hamazaki, Shigeo Murata
  • Organizer: FASEB Science Research Conferences 2014 Ubiquitin and Cellular Regulation
  • Place of Presentation: Saxton River, Vermont, USA
  • Year and Date: 2014-06-15 – 2014-06-20
• [Presentation] プロテアソーム会合チオレドキシン様分子のメラニン産生への関与 (2013)
  • Author(s): 上地浩之、濱崎純、村田茂穂
  • Organizer: 新学術領域研究 ユビキチンネオバイオロジー 平成25年度第2回領域班会議
  • Place of Presentation: 静岡、熱海
• [Presentation] Sirt1によるHsp70を介したタンパク質品質管理 (2013)
  • Author(s): 冨田拓哉、濱崎純、平山尚志郎、八代田英樹、村田茂穂
  • Organizer: 新学術領域研究 ユビキチンネオバイオロジー 平成25年度第2回領域班会議
  • Place of Presentation: 静岡、熱海
• [Presentation] Testis-specific proteasome and its role in mammalian testes (2013)
  • Author(s): Hiroyuki Uechi, Jun Hamazaki, Shigeo Murata
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 神奈川、横浜
• [Presentation] Genetic analysis of novel mammalian protesasome subunit Rpn13 (2013)
  • Author(s): Jun Hamazaki, Shigeo Murata
  • Organizer: The 35th NAITO CONFERENCE ON The Ubiquitin-Proteasome System
  • Place of Presentation: 北海道、札幌
• [Presentation] Transcriptional Modulators of the Mammalian 26S Proteasome – _A Genome-wide Perspective (2013)
  • Author(s): Kogleck Larissa, Hirayama Shoshiro, Hamazaki Jun, Murata Shigeo
  • Organizer: 東京大学生命科学シンポジウム
  • Place of Presentation: 東京、本郷
• [Presentation] Functional Analysis of PI31 (2013)
  • Author(s): Haruta Yoshitaro, Hamazaki Jun, Murata Shigeo
  • Organizer: 東京大学生命科学シンポジウム
  • Place of Presentation: 東京、本郷
• [Presentation] Genetic analysis of novel mammalian proteasome subunit Rpn13 (2012)
  • Author(s): Jun Hamazaki, Shigeo Murata
  • Organizer: FEBS Special Meeting on Protein Quality Control and Ubiquitin Proteasome System in Health and Disease
  • Place of Presentation: Izmir (Turkey)
• [Presentation] A genome-wide approach to identify the regulatory mechanisms of mammalian 26S proteaome gene transcription (2012)
  • Author(s): Larissa Kogleck, Jun Hamazaki, Shigeo Murata
  • Organizer: FEBS Special Meeting on Protein Quality Control and Ubiquitin Proteasome System in Health and Disease
  • Place of Presentation: Izmir (Turkey)
• [Presentation] Regulationo fo the ubiquitin-proteasome system by Sirt1
  • Author(s): Takuya Tomita, Jun Hamazaki, Hideki Yashiroda, Shigeo Murata
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Functional analysis of PI31
  • Author(s): Takaya Ishii, Jun Hamazaki, Shigeo Murata
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Investigation of the biological meaning of b subunit diversity
  • Author(s): Akihito Fukuda, Jun Hamazaki, Shigeo Murata
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Enzymatic purification and mass spectrometric identification of polyubiquitinated proteins
  • Author(s): Naoyuki Mita, Kiyo Shimada, Takeumi Kaneko, Jun Hamazaki, Shigeo Murata
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Genetic analysis of the ubiquitin receptor subunit of the proteasome, Rpn13 in mice
  • Author(s): Jun Hamazaki, Shigeo Murata
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Book] 医学のあゆみ、活性酸素・基礎から病態解明・制御まで (2013)
  • Author(s): 濱崎純、村田茂穂
  • Total Pages: 6
  • Publisher: 医歯薬出版
• [Book] 実験医学増刊 活性酸素・ガス状分子による恒常性制御と疾患 (2013)
  • Author(s): 濱崎 純、村田 茂穂
  • Publisher: 羊土社