| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Outline of Final Research Achievements |
The identification of D-aspartate (D-Asp) synthesizing enzyme, mammalian Asp racemase was conducted. All trials were unsuccessful to prepare the recombinant proteins with the candidate genes in E. coli cells, since the proteins were all recovered in the insoluble fractions. Therefore, the issue was addressed whether the D-Asp synthesis was influenced by small interference RNA (siRNA) of the candidate genes. The suppression of a gene (Got1l1), a candidate gene reported by an American research group had no influence on the production of D-Asp in the cultured mammalian cells those can synthesize D-Asp. The gene Got1l1 is presumably uninvolved in the biosynthesis of D-Asp in the mammalian cells.
|
