| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Oxidized low-density lipoprotein (oxLDL) present in vivo, which is involved in atherogenesis, has not been well characterized. In this study, we introduced LC-MS/MS procedure to quantify various oxidized phosphatidylcholine (oxPC) and lysoPC species with high sensitivity. With this technique we elucidated molecular species of oxPC and lysoPC produced during LDL oxidation process. In addition, we successfully separated in vivo oxLDL from human plasma for the first time using an anion-exchange column and a monoclonal antibody against oxidized PC. The in vivo oxLDL was revealed to be a particle with anionic charges and modification with oxPC. oxLDL could potentially be a marker to evaluate oxidative stresses in systemic diseases and/or local tissues.
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