Molecular basis of the avian memory formation regulated by cytoskelton binding protein

• Project/Area Number: 24590096
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Teikyo University
• Principal Investigator: YAMAGUCHI Shinji 帝京大学, 薬学部, 准教授 (60398740)
• Co-Investigator(Renkei-kenkyūsha): AOKI Naoya 帝京大学, 薬学部, 助教 (60525334)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 生化学 / 行動生化学 / 分子生物学 / 記憶 / 刻印付け

Outline of Final Research Achievements

Birds have a diverse behavioral repertoire and are thus ideal models for experimental analyses of neural and behavioral plasticity. The domestic chick has been intensively studied as a model for filial imprinting. To elucidate the molecular processes underlying the neural mechanism of filial imprinting in newly-hatched chicks more extensively, we previously conducted a cDNA microarray and the quantitative RT-PCR study. One of the genes was microtubule-associated protein 2 (MAP2), which is involved in cytoskeletal organization. Here, we used in vivo gene-transfer technique and showed that transfected microRNA vectors preferentially silence exogenous DNA expression in neuronal cells. Using this system, the up-regulation of MAP2 accompanying filial imprinting was suppressed in vivo, which impaired the filial imprinting in chicks. Our results suggest that the regulation of MAP2 expression is required for filial imprinting.

Research Products

• [Journal Article] Expression of the voltage-sensing phosphatase gene in the chick embryonic tissues and in the adult cerebellum. (2014)
  • Author(s): Yamaguchi, S., Aoki, N., Kitajima, T., Okamura, Y. and Homma, K. J.
  • Journal Title: Communicative & Integrative Biology Volume: 7 Issue: 5 Pages: 1-4
  • DOI: 10.4161/19420889.2014.970502
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Potential role of voltage-sensing phosphatases in regulation of cell structure through the production of PI(3,4)P2 (2013)
  • Author(s): Yamaguchi S, Kurokawa T, Taira I, Aoki N, Sakata S, Okamura Y & Homma KJ
  • Journal Title: J. Cell Physiol. Volume: 229(4) Issue: 4 Pages: 422-33
  • DOI: 10.1002/jcp.24463
  • Peer Reviewed / Open Access
• [Journal Article] A primer for learning: Thyroid hormone determines the start of the sensitive period of filial imprinting of domestic chicks (2013)
  • Author(s): homma, K.J., Yamaguchi,S. Y., Aoki, N.
  • Journal Title: Seikagaku Volume: 85(5) Pages: 315-327
  • Peer Reviewed
• [Journal Article] 鳥類刷り込みに見られるメモリープライミング (2013)
  • Author(s): 本間 光一、山口 真二、青木 直哉
  • Journal Title: 分子精神医学 Volume: 13（４） Pages: 243-250
  • Peer Reviewed
• [Journal Article] Thyroid hormone determines the start of the sensitive period of imprinting and primes later learning (2012)
  • Author(s): Yamaguchi, S., Aoki, N., Kitajima, T.Iikubo, E., Katagiri, S., Matsushima,T., Homma,K.J.
  • Journal Title: Nature Communications Volume: 3 Issue: 1 Pages: 1081-1081
  • DOI: 10.1038/ncomms2088
  • URL: http://dx.doi.org/10.1016/j.anbehav.2012.07.026
  • Peer Reviewed
• [Journal Article] 3' phosphatase activity toward PI(3,4)P2 by voltage-sensing phosphatase (2012)
  • Author(s): Kurokawa T, Takasuga S, Sakata S, Yamaguchi S, Horie S, Homma KJ, Sasaki T & Okamura Y
  • Journal Title: VSP. Proc. Natl. Acad. Sci. U.S.A. Volume: 109(25) Issue: 25 Pages: 10089-94
  • DOI: 10.1073/pnas.1203799109
  • Peer Reviewed
• [Presentation] 鳥類刻印付けにおけて大脳背側部IMHAは獲得・想起に必要である (2015)
  • Author(s): 青木直哉、山口真二、北島孝明、本間光一
  • Organizer: 第67回日本動物学会関東支部大会
  • Place of Presentation: 早稲田大学先端生命医科学センター
  • Year and Date: 2015-03-14
• [Presentation] 甲状腺ホルモンは、刷り込み学習の感受性期の開始を決定し、その後の学習のプライマーとして働く (2014)
  • Author(s): 山口真二、青木直哉、松島俊也、本間光一
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] 鳥類刻印付けの成立に大脳後背側部IMHAは必須である (2014)
  • Author(s): 青木直哉、北島孝明、松島俊也、本間光一
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] Hormonal regulation of the sensitive period for fillial imprinting in domestic chicks. (2014)
  • Author(s): 山口真二、青木直哉、本間光一
  • Organizer: 2014 International Congress of Neuroethology,Hokkaido Neuroethology workshops
  • Place of Presentation: 北海道大学理学部生命科学科
  • Year and Date: 2014-07-26 – 2014-07-27
• [Presentation] Neural circuits of the sensitive period triggered by thyroid hormones in filial imprinting. (2014)
  • Author(s): 青木直哉、山口真二、本間光一
  • Organizer: 2014 International Congress of Neuroethology,Hokkaido Neuroethology workshops
  • Place of Presentation: 北海道大学理学部生命科学科
  • Year and Date: 2014-07-26 – 2014-07-27
• [Presentation] Imprinting behavior in birds as a model of learning potency and the sensitive period (2014)
  • Author(s): Yamaguchi, S
  • Organizer: Gordon Research Conference, Genes & Behavior
  • Place of Presentation: Hotel Galvez in Galveston, TX, USA
• [Presentation] 鳥類刻印付け感受性期の開始を決定するメモリープライミング (2013)
  • Author(s): 山口真二、青木直哉、北島孝明、松島俊也、本間光一
  • Organizer: Neuro2013(第36回日本神経科学大会）
  • Place of Presentation: 国立京都国際会館
• [Presentation] Thyroid hormone determines the start of the sensitive period of visual imprinting
  • Author(s): S. YAMAGUCHI, N. AOKI, T. KITAJIMA, T. MATSUSHIMA, K. J. HOMMA
  • Organizer: Society for Neuroscience 2012
  • Place of Presentation: New Orleans
• [Presentation] Rapid inflow of thyroid hormone induced by visual imprinting primes later learning.
  • Author(s): N. AOKI, S. YAMAGUCHI, T. KITAJIMA, T. MATSUSHIMA, K. J. HOMMA
  • Organizer: Society for Neuroscience 2012
  • Place of Presentation: New Orleans
• [Presentation] 甲状腺ホルモンは鳥類親子刷り込みの感受性期の開始を決定する
  • Author(s): 本間 光一、山口真二、青木直哉、松島俊也
  • Organizer: 日本動物学会 第８３回大会 ２０１２大阪
  • Place of Presentation: 大阪
• [Book] Insect papain homologues (In Handbook of Proteolytic Enzyme, 3rd Edition ( A. J. Barrett, N. D. Rawlings, and J. D. Woessner, eds) ) (2012)
  • Author(s): Homma, K. J. , Yamaguchi, S. and Aoki, N.
  • Total Pages: 6
  • Publisher: Academic Press
• [Remarks] 帝京大学薬学部ホームページ
  • URL: http://www.pharm.teikyo-u.ac.jp/
• [Remarks] 帝京大学教員情報
  • URL: https://www.e-campus.gr.jp/staffinfo/public/staff/detail/1303/16
• [Remarks] 帝京大学薬学部ホームページ
  • URL: http://www.pharm.teikyo-u.ac.jp/
• [Remarks] 帝京大学教員情報
  • URL: https://www.e-campus.gr.jp/staffinfo/public/staff/detail/1303/16
• [Remarks] 帝京大学ホームページ
  • URL: http://www.teikyo-u.ac.jp/