| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
To clarify the transition mechanisms of calreticulin (CRT) from ER to cell surface in anti-cancer drug-treated tumor cells, the expression levels of CRT on the plasma membranes of mitoxantrone or oxaliplatin -treated human colon cancer cell line HT29 cells were estimated. It revealed that the CRT on the cell membrane exhibits increased biphasic at the protein level, and the increase of early phase was result by ER stress, ROS and activation of caspase8, the increase of late phase was concerned with apoptosis. Furthermore, mAG1-labelled CRT transfected HLF cells (HLF-ER-mAG1-CRT-KDEL cells) were prepared, and analyzed by confocal laser microscope for localizes changes of CRT. It was confirmed that mAG1-CRT were aggregated and translocated from the ER to plasma membrane, in mitoxantrone or ER stress -inducing agents thapsigargin treated HLF-ER-mAG1-CRT-KDEL cells.
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