| Project/Area Number |
24590107
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Tokyo Metropolitan Institute of Medical Science |
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Principal Investigator |
KITAMURA Noriko 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 研究員 (80415603)
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| Co-Investigator(Kenkyū-buntansha) |
KAMINUMA Osamu 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 主任研究員 (80342921)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | NFAT / CN / 免疫抑制薬 / 新規免疫抑制薬 |
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| Outline of Final Research Achievements |
Since numerous side effects of CN inhibitors are mostly caused by the diversity of the NFAT family, control of a part, not all, of the function of NFAT family members is desirable. By comparing the binding activity of CN with each NFAT, we identified a new CN-binding region into NFATc1. The CN-binding affinity of this region was much different among the NFAT family. By means of competition assay using sequential partial peptides, we determined the core peptide and essential amino acids in it. By analyzing the gene expression in NFATc4-siRNA expressing cells, and the NFATc4 promoter activity in siRNA library-transfected cells, several candidate molecules related with the selectivity among NFAT family members were identified. The molecular mechanisms useful for the regulation of a part of NFAT family members were clarified.
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