Regulatory mechanism of P2X7 receptor by its splice variants, and relationship of its altered functional expression to psychiatric diseases
| Project/Area Number |
24590128
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Kyoto Pharmaceutical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NISHIDA Kentaro 京都薬科大学, 薬学部, 助教 (20533805)
松尾 剛明 京都薬科大学, 薬学部, 助教 (60612702)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ATP / 脳・神経 / 受容体 / P2X7 / アストロサイト / スプライスバリアント / 食作用 |
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| Outline of Final Research Achievements |
The activity of P2X7 receptors expressed in cultured astrocytes of SJL-strain mice, which exhibit extremely aggression characteristics, was greater than the case of ddY-strain mice, and this was due to the less expression levels of P2X7 receptor splice variant 2 and 3 in the formers. In addition, we demonstrated that spontaneous activation of P2X7Rs expressed by astrocytes plays a role in regulation of engulfing activity of astrocytes. Finally, we found that in activated astrocytes, which was induced by stress-load such as oxidative stress, expression of P2X7 receptors at the plasma membrane was decreased by increased expression of its splice variant 2 and 3, resulting in decreased engulfing activity. These findings indicated that P2X7 receptor is regulated by expression levels of its splice variants, and its activity plays a role in regulation of astrocytic functionality.
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Report
(4 results)
Research Products
(47 results)
