A Drug Discovery-study targeting the transcriptional factor, NF-KappaB

• Project/Area Number: 24590150
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Drug development chemistry
• Research Institution: Nigata University of Phermacy and Applied Life Sciences
• Principal Investigator: ASADA Shinichi 新潟薬科大学, 薬学部, 助教 (50424883)
• Co-Investigator(Kenkyū-buntansha): KITAGAWA Kouki 新潟薬科大学, 薬学部, 教授 (60093853)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 転写因子 / 核内調節因子 / ペプチド / NF-KappaB / アフィニティー精製 / 転写調節因子 / ペプチド合成 / ケミカルバイオロジー / NF-kB / 化学合成ペプチド / ペプチドプローブ

Outline of Final Research Achievements

We have aimed to obtain the small molecules that regulate the nuclear transcriptional factor, NF-kappaB, consequently find out the lead molecules for the preventive drugs of arteriosclerosis. The chemically synthesized affinity peptide probes that mimic the transactivation domains of NF-kappaB p65 were planned to identify the nuclear co-activator for the NF-kappaB. However, the yield of the chemically synthesized peptide was too low to utilize as an affinity probe, recombinant peptide was used for ths probe alternatively. Using probe this recombinant NF-kappaBp65 TA2 region as a probe, a novel protein specifically interact with this region was found by an affinity pull-down assay and identified. Further analysis is proceeding to reveal the dynamics of this interaction and to reach the goal of this study.

Research Products

• [Remarks] 薬品製造学研究室
  • URL: http://yakuhinseizou.nupals.ac.jp