| Project/Area Number |
24590150
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Drug development chemistry
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| Research Institution | Nigata University of Phermacy and Applied Life Sciences |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KITAGAWA Kouki 新潟薬科大学, 薬学部, 教授 (60093853)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 転写因子 / 核内調節因子 / ペプチド / NF-KappaB / アフィニティー精製 / 転写調節因子 / ペプチド合成 / ケミカルバイオロジー / NF-kB / 化学合成ペプチド / ペプチドプローブ |
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| Outline of Final Research Achievements |
We have aimed to obtain the small molecules that regulate the nuclear transcriptional factor, NF-kappaB, consequently find out the lead molecules for the preventive drugs of arteriosclerosis. The chemically synthesized affinity peptide probes that mimic the transactivation domains of NF-kappaB p65 were planned to identify the nuclear co-activator for the NF-kappaB. However, the yield of the chemically synthesized peptide was too low to utilize as an affinity probe, recombinant peptide was used for ths probe alternatively. Using probe this recombinant NF-kappaBp65 TA2 region as a probe, a novel protein specifically interact with this region was found by an affinity pull-down assay and identified. Further analysis is proceeding to reveal the dynamics of this interaction and to reach the goal of this study.
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