Development of an in vitro allergenicity test metod for drugs considered metabolic activation

Research Project

Project/Area Number	24590157
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Drug development chemistry
Research Institution	Tokyo University of Marine Science and Technology (2013-2014) National Institute of Health Sciences (2012)
Principal Investigator	KUROSE Kouichi 東京海洋大学, 海洋科学技術研究科, 教授 (30280754)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000) Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	インビトロ試験法 / 薬物アレルギー / 感作性
Outline of Final Research Achievements	Using human monocytic cell line THP-1, we developed a simple and high-throughput method to evaluate drug allergenicity in vitro, which is utilizing real-time RT-PCR. In addition, we established the cell lines, from THP-1, stably expressing a representative drug metabolizing enzyme CYP3A4, which is expected to be utilized for our new method above mentioned.

Report

(4 results)