The functional regulation of metallothionein by functional organic-inorganic hybrid molecules and its molecular mechanism
| Project/Area Number |
24590163
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental pharmacy
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| Research Institution | Tokyo University of Pharmacy and Life Science (2014)
Aichi Gakuin University (2012-2013) |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
UCHIYAMA Masanobu 東京大学, 薬学部, 教授 (00271916)
YASUIKE Shuji 愛知学院大学, 薬学部, 教授 (10230210)
NAKA Hiroshi 名古屋大学, 物質科学国際研究センター, 助教 (70431517)
LEE Jin-Yong 愛知学院大学, 薬学部, 講師 (80581280)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 薬学 / 有機金属化合物 / 錯体分子 / メタロチオネイン / 血管内皮細胞 / 生体防御因子 / 動脈硬化 |
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| Outline of Final Research Achievements |
In the present study, out of 120 compounds, we found that Cu(II)(Edtc)2 (Cu10), a organic-inorganic hybrid molecule, strongly induces metallothionein (MT) synthesis without non specific cell damage in human vascular endothelial cells. We also showed that Cu10 is easily taken up by vascular endothelial cells, and that Cu10 induce MT synthesis via metal response element-binding transcription factor-1 (MTF-1) pathway but not Nrf-2 pathway. Cu10 also induces MT synthesis in other cell types such as human brain microvascular pericytes and human coronary artery smooth muscle cells. However, the stimulatory effect of Cu10 was weaker than Cu10-stimulated vascular endothelial cells, suggesting that Cu10 has more strong activity on MT synthesis in vascular endothelial cells. These observations suggest that Cu10, a organic-inorganic hybrid molecule, may have a potential to contribute for prevention of vascular diseases including atherosclerosis.
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Report
(4 results)
Research Products
(14 results)
