Comparative genome analysis of nontuberculous mycobacteria and its clinical application
Project/Area Number |
24590164
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Environmental pharmacy
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Research Institution | Meijo University |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | Mycobacterium avium / 肺M. avium症 / ゲノム解析 / 新規プラスミド / 感染様式 / MAC症 / 比較ゲノム / プラスミド / M. avium |
Outline of Final Research Achievements |
To explore the bacterial factors that affect the pathological state of disease caused by Mycobacterium avium, we determined the complete genome sequence of strain TH135 isolated from a patient with pulmonary M. avium disease, and further demonstrated the presence of a novel plasmid, pMAH135. This plasmid consists of 194,711 nucleotides and has 164 coding sequences, some of which encode proteins involved in the pathogenicity of mycobacteria and their resistance to antimicrobial agents. The screening of M. avium isolates from humans and pigs for genes located on pMAH135 revealed that these genes are more commonly detected in isolates from patients with pulmonary M. avium disease than in HIV-positive patients. However, the genes were almost entirely absent in isolates from pigs, suggesting that pMAH135 influences not only the pathological manifestations of M. avium disease, but also the host specificity.
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Report
(4 results)
Research Products
(32 results)
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[Journal Article] Characterization of a novel plasmid, pMAH135, from Mycobacterium avium subsp. hominissuis2015
Author(s)
Kei-ichi Uchiya, Hiroyasu Takahashi, Taku Nakagawa, Tetsuya Yagi, Makoto Moriyama, Takayuki Inagaki, Kazuya Ichikawa, Toshiaki Nikai, Kenji Ogawa
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Journal Title
PLoS One
Volume: VOL.10
Issue: 2
Pages: e0117797-e0117797
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] X-ray structure analysis and characterization of AFUEI, an elastase inhibitor from Aspergillus fumigatus2013
Author(s)
Mayuko Sakuma, Katsumi Imada, Yoshiyuki Okumura, Kei-ichi Uchiya, Nobuo Yamashita, Kenji Ogawa, Atsushi Hijikata, Tsuyoshi Shirai, Michio Homma, Toshiaki Nikai
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Journal Title
J. Biol. Chem.
Volume: 288
Issue: 24
Pages: 17451-17459
DOI
Related Report
Peer Reviewed
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