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Comparative genome analysis of nontuberculous mycobacteria and its clinical application

Research Project

Project/Area Number 24590164
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental pharmacy
Research InstitutionMeijo University

Principal Investigator

UCHIYA Kei-ichi  名城大学, 薬学部, 准教授 (70168714)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsMycobacterium avium / 肺M. avium症 / ゲノム解析 / 新規プラスミド / 感染様式 / MAC症 / 比較ゲノム / プラスミド / M. avium
Outline of Final Research Achievements

To explore the bacterial factors that affect the pathological state of disease caused by Mycobacterium avium, we determined the complete genome sequence of strain TH135 isolated from a patient with pulmonary M. avium disease, and further demonstrated the presence of a novel plasmid, pMAH135. This plasmid consists of 194,711 nucleotides and has 164 coding sequences, some of which encode proteins involved in the pathogenicity of mycobacteria and their resistance to antimicrobial agents. The screening of M. avium isolates from humans and pigs for genes located on pMAH135 revealed that these genes are more commonly detected in isolates from patients with pulmonary M. avium disease than in HIV-positive patients. However, the genes were almost entirely absent in isolates from pigs, suggesting that pMAH135 influences not only the pathological manifestations of M. avium disease, but also the host specificity.

Report

(4 results)
  • 2015 Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (32 results)

All 2015 2014 2013 2012 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (20 results) (of which Invited: 1 results) Book (1 results)

  • [Journal Article] Characterization of a novel plasmid, pMAH135, from Mycobacterium avium subsp. hominissuis2015

    • Author(s)
      Kei-ichi Uchiya, Hiroyasu Takahashi, Taku Nakagawa, Tetsuya Yagi, Makoto Moriyama, Takayuki Inagaki, Kazuya Ichikawa, Toshiaki Nikai, Kenji Ogawa
    • Journal Title

      PLoS One

      Volume: VOL.10 Issue: 2 Pages: e0117797-e0117797

    • DOI

      10.1371/journal.pone.0117797

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Recurrence of pulumonary Mycobacterium avium complex disease due to endogenous reactivation2014

    • Author(s)
      Shu Taga, Tetsuya Yagi, Kei-ichi Uchiya, Yuichi Shibata, Hiromitsu Hamaura, Yaku Nakagawa, Yuta Hayashi, Toshiaki Nikai, and Kenji Ogawa
    • Journal Title

      JMM Case Reports

      Volume: VOL.1 Pages: 1-4

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Okinalysin, a novel P–I metalloproteinase from Ovophis okinavensis: biological properties and effect on vascular endothelial cells2014

    • Author(s)
      Yumiko Komori, Eri Murakami, Kei-ichi Uchiya, Tunemasa Nonogaki and Toshiaki Nikai
    • Journal Title

      Toxins

      Volume: VOL.6 Pages: 2594-2604

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparative genome analysis of Mycobacterium avium revealed genetic diversity in strains that cause pulmonary and disseminated disease2013

    • Author(s)
      Kei-ichi Uchiya, Hiroyasu Takahashi, Yetsuya yagi, Makoto Moriyama, Takayuki Inagaki, Kazuya Ichikawa, Taku Nakagawa, Toshiaki Nikai, Kenji Ogawa
    • Journal Title

      PLoS One

      Volume: 8 Issue: 8 Pages: e71831-e71831

    • DOI

      10.1371/journal.pone.0071831

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] X-ray structure analysis and characterization of AFUEI, an elastase inhibitor from Aspergillus fumigatus2013

    • Author(s)
      Mayuko Sakuma, Katsumi Imada, Yoshiyuki Okumura, Kei-ichi Uchiya, Nobuo Yamashita, Kenji Ogawa, Atsushi Hijikata, Tsuyoshi Shirai, Michio Homma, Toshiaki Nikai
    • Journal Title

      J. Biol. Chem.

      Volume: 288 Issue: 24 Pages: 17451-17459

    • DOI

      10.1074/jbc.m112.433987

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterization and identification of partial amino acid sequence of a novel elastase inhibitor, asnidin from Aspergillus nidulans2013

    • Author(s)
      Yoshiyuki Okumura, Makoto Suzukawa, Kei-ichi Uchiya, Kenji Ogawa, Yumiko Komori, Nobuo Yamashita, Toshiaki Nikai
    • Journal Title

      Med. Mycol. J.

      Volume: 54 Pages: 279-284

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] 初回肺結核治療中において短期間に多剤耐性化した結核菌の遺伝子解析—臨床分離株を用いて—2013

    • Author(s)
      稲垣孝行、中川 拓、前田伸司、村瀬良朗、市川和哉、森山 誠、打矢惠一、八木哲也、二改俊章、小川賢二
    • Journal Title

      Kekkaku

      Volume: 88 Pages: 595-604

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] 肺MAC症患者由来株と韓国人肺MAC症患者由来株の遺伝学的特徴の違い2012

    • Author(s)
      新美政樹、打矢惠一、八木哲也、髙橋弘泰、黒河和宏、市川和哉、稲垣孝行、森山 誠、二改俊章、林 悠太、中川 拓、小川賢二
    • Journal Title

      Kekkaku

      Volume: 87 Pages: 461-467

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mycobcterium aviumの新規Variable number tandem repeat領域の有用性の検討2012

    • Author(s)
      黒河和宏、打矢惠一、八木哲也、髙橋弘泰、新美政樹、市川和哉、稲垣孝行、森山 誠、二改俊章、林 悠太、中川 拓、小川賢二
    • Journal Title

      Kekkaku

      Volume: 87 Pages: 491-499

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 肺MAC症患者由来株と韓国人肺MAC症患者由来株の遺伝学的特徴の違い2012

    • Author(s)
      中川 拓、髙橋弘泰、市川和哉、稲垣孝行、打矢惠一、二改俊章、八木哲也、小川賢二
    • Journal Title

      Kekkaku

      Volume: 87 Pages: 687-695

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Salmonella: strategies for survival2012

    • Author(s)
      Kei-ichi Uchiya and Toshiaki Nikai
    • Journal Title

      Toxin Reviews

      Volume: 31 Pages: 1-10

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] セレウス菌の嘔吐毒セレウリドの毒力はカチオン比に影響を受ける2015

    • Author(s)
      岡本 陽、藪谷充孝、打矢惠一、二改俊章
    • Organizer
      第88回日本細菌学会総会
    • Place of Presentation
      岐阜
    • Year and Date
      2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] コンタクトレンズの適正使用に関する調査2015

    • Author(s)
      小森由美子、櫻井梨絵、鎌倉里恵、多湖美登里、石川泰成、打矢惠一、二改俊章
    • Organizer
      日本薬学会第135年会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 肺MAC症患者由来のゲノム解析と病原因子の検討2015

    • Author(s)
      打矢惠一
    • Organizer
      第90回日本結核病学会総会
    • Place of Presentation
      長崎
    • Year and Date
      2015-03-17
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 麹菌 A. oryzaeのエラスターゼインヒビター様タンパク質2014

    • Author(s)
      山下伸雄、平松穂乃花、奥村欣由、小森由美子、打矢惠一、明石貴裕、小川賢二、二改俊章
    • Organizer
      第14回糸状菌分子生物学コンファレンス
    • Place of Presentation
      仙台
    • Year and Date
      2014-11-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] 肺MAC症患者由来、Mycobacterium aviumが保有する新規プラスミド2014

    • Author(s)
      打矢惠一、小川賢二、二改俊章
    • Organizer
      第51回日本細菌学会中部支部総会
    • Place of Presentation
      金沢
    • Year and Date
      2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] コンタクトレンズの適正使用に関する調査2014

    • Author(s)
      櫻井梨絵、鎌倉里恵、多湖美登里、小森由美子、打矢惠一、石川泰成、二改俊章
    • Organizer
      第60回日本薬学会東海支部総会・大会
    • Place of Presentation
      鈴鹿
    • Year and Date
      2014-07-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] 肺MAC症患者由来および自宅浴室由来MAC菌分離株のvariable–number tandem–repeat (VNTR) 型別解析パターンに関する検討2014

    • Author(s)
      多賀 収、打矢惠一、酒井紫乃、橘 史緒、二改俊章、小川賢二
    • Organizer
      第88回日本感染症学会学術講演会・第62回日本化学療法学会総会合同学会
    • Place of Presentation
      福岡
    • Year and Date
      2014-06-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] X-ray structure analysis of AFUEI, an elastase inhibitor from Aspergillus fumigatus2013

    • Author(s)
      Mayuko Sakuma, Katsumi Imada, Yoshiyuki Okumura, Kei-ichi Uchiya, Atsushi Hijikata, Tsuyoshi Shirai, Michio Homma, and Toshiaki Nikai
    • Organizer
      4th International Symposium on Diffraction Structural Biology
    • Place of Presentation
      名古屋
    • Related Report
      2013 Research-status Report
  • [Presentation] 肺MAC症患者からのMycobacterium aviumのゲノム解析2013

    • Author(s)
      柴田祐一、打矢惠一、森山 誠、小川賢二、二改俊章
    • Organizer
      第50回日本細菌学会中部支部総会
    • Place of Presentation
      蒲郡
    • Related Report
      2013 Research-status Report
  • [Presentation] Structural similarity of AFUEI, an elastase inhibitor from Aspergillus fumigatus, and the potato I family inhibitors from plants2013

    • Author(s)
      Mayuko Sakuma, Katsumi Imada, Yoshiyuki Okumura, Kei-ichi Uchiya, Atsushi Hijikata, Tsuyoshi Shirai, Toshiaki Nikai, and Michio Homma
    • Organizer
      第51会日本生物物理学会年会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Presentation] VNTR (Variable Number Tandem Repeat) 型別解析及びプロミクスNTMを用いたMycobacterium aviumの喀痰及び環境分離株の性質の検討2013

    • Author(s)
      柴田祐一、多賀 収、打矢惠一、森山 誠、稲垣孝行、八木哲也、市川和哉、林 悠太、二改俊章、小川賢二
    • Organizer
      第67回国立病院総合医学会
    • Place of Presentation
      金沢
    • Related Report
      2013 Research-status Report
  • [Presentation] Mycobacterium aviumのVariable Number Tandem Repeat型別解析と薬剤感受性との関係2013

    • Author(s)
      濱浦弘光、打矢惠一、森山 誠、稲垣孝行、八木哲也、市川和哉、中川 拓、二改俊章、小川賢二
    • Organizer
      第67回国立病院総合医学会
    • Place of Presentation
      金沢
    • Related Report
      2013 Research-status Report
  • [Presentation] Comparative genome analysis of Mycobacterium avium strains that cause different MAC disease2013

    • Author(s)
      Kei-ichi Uchiya, Toshiaki Niaki
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Presentation] 麹菌Aspergillus oryzaeのエラスターゼインヒビター様タンパク質の単離2013

    • Author(s)
      山下伸雄、奥村欣由、打矢惠一、小川賢二、鶴田宏樹、明石貴裕、二改俊章
    • Organizer
      2014年度 日本農芸化学会大会
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Presentation] Mycobacterium avium の分子疫学解析法の臨床における有用性の検討

    • Author(s)
      田中映美、打矢惠一、森山誠、小川賢二、二改俊章
    • Organizer
      第58回日本薬学会東海支部大会
    • Place of Presentation
      静岡
    • Related Report
      2012 Research-status Report
  • [Presentation] 臨床使用消毒薬検出菌における消毒薬耐性の検討

    • Author(s)
      中村博厚、森山誠、打矢惠一、二改俊章
    • Organizer
      第58回日本薬学会東海支部大会
    • Place of Presentation
      静岡
    • Related Report
      2012 Research-status Report
  • [Presentation] サルモネラの病原性における細胞内カルシウムイオンの重要性

    • Author(s)
      打矢惠一、二改俊章
    • Organizer
      フォーラム2012衛生薬学・環境トキシコロジー
    • Place of Presentation
      名古屋
    • Related Report
      2012 Research-status Report
  • [Presentation] MmpL遺伝子が関与する肺MAC症治療薬の薬剤耐性

    • Author(s)
      福大哉、打矢惠一、二改俊章、田中映美、林悠太、中川拓、山田憲隆、垂水修、長谷川万里子、小川賢二
    • Organizer
      第66回国立病院総合医学会
    • Place of Presentation
      神戸
    • Related Report
      2012 Research-status Report
  • [Presentation] 肺Mycobacterium avium complex症におけるVariable Number Tandem Repeat型別解析法の臨床への応用

    • Author(s)
      田中映美、福大哉、打矢惠一、二改俊章、長谷川万里子、林悠太、垂水修、中川拓、山田憲隆、小川賢二
    • Organizer
      第66回国立病院総合医学会
    • Place of Presentation
      神戸
    • Related Report
      2012 Research-status Report
  • [Presentation] Mycobacterium aviumのmmpL遺伝子と薬剤耐性との関係

    • Author(s)
      濱浦弘光、福大哉、打矢惠一、田中映美、森山誠、二改俊章
    • Organizer
      第49回日本細菌学会中部支部総会
    • Place of Presentation
      金沢
    • Related Report
      2012 Research-status Report
  • [Book] 肺MAC症診療 Up to Date -非結核性抗酸菌症のすべて-2013

    • Author(s)
      打矢惠一(分担)
    • Total Pages
      2
    • Publisher
      南江堂
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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