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Analysis of transport mechanism of cadmium and manganese in their target organs

Research Project

Project/Area Number 24590168
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental pharmacy
Research InstitutionTokushima Bunri University

Principal Investigator

FUJISHIRO Hitomi  徳島文理大学, 薬学部, 助教 (10389182)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsカドミウム / マンガン / 輸送 / 腎臓 / 神経 / 消化管 / 中毒学 / 金属 / ZIP8 / ZIP14 / 亜鉛輸送体 / 毒性
Outline of Final Research Achievements

The purpose of this study was to clarify the mechanism of Cd and Mn transport in the target organs of their toxicity. To examine Cd transport in the proximal tubule of kidney, we obtained immortalized cells derived from S1, S2, and S3 segments of mouse proximal tubule. We found that different patterns of Cd uptake and excretion among segment-specific proximal tubule epithelial cells.
We demonstrated that not ZIP14 which is inducible by cytokines, might play an important role in the uptake of Mn in neuronal cells. The inflammatory cytokine enhanced the accumulation of Mn to neuronal cells via the increase in Mn uptake and the decrease in Mn excretion. These data suggest that metal transporters might play an important role in accumulation of Mn in the brain of neurodegenerative diseases where the levels of inflammatory cytokine are abnormally elevated.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (34 results)

All 2015 2014 2013 2012 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (23 results) (of which Invited: 15 results) Remarks (5 results)

  • [Journal Article] Quantum dots induce heat shock-related cytotoxicity at intracellular environment.2014

    • Author(s)
      Migita, S., Moquin, A., Fujishiro, H., Himeno, S., Maysinger, D., Winnik, F. M., Taniguchi, A.
    • Journal Title

      In Vitro Cell Dev Biol Anim.

      Volume: 50(4) Issue: 4 Pages: 367-372

    • DOI

      10.1007/s11626-013-9693-2

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Interleukin-6 enhances manganese accumulation in SH-SY5Y cells: Implications of the up-regulation of ZIP14 and the down-regulation of ZnT10.2014

    • Author(s)
      Fujishiro, H., Yoshida, M., Nakano, Y., Himeno, S.
    • Journal Title

      Metallomics

      Volume: 6 Issue: 4 Pages: 944-949

    • DOI

      10.1039/c3mt00362k

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Suppression of ZIP8 expression is a common feature of cadmium-resistant andmanganese-resistant RBL-2H3 cells.2013

    • Author(s)
      Fujishiro, H., Ohashi, T., Takuma, M., Himeno, S.
    • Journal Title

      Metallomics

      Volume: 5 Issue: 5 Pages: 437-444

    • DOI

      10.1039/c3mt00003f

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Down-regulation of S100A9 and S100A10 in manganese-resistant RBL-2H3 cells.2013

    • Author(s)
      Fujishiro, H., Ohashi, T., Takuma, M., Himeno, S.
    • Journal Title

      J. Toxicol. Sci.

      Volume: 38(5) Pages: 753-757

    • NAID

      10031191734

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Suppression of ZIP8 expression is a common feature of cadmium-resistant and manganese-resistant RBL-2H3 cells2013

    • Author(s)
      Fujishiro, H., Ohashi, T., Takuma, M., Himeno, S.
    • Journal Title

      Metallomics

      Volume: in press

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Roles of ZIP8, ZIP14, and DMT1 in transport of cadmium and manganese in mouse kidney proximal tubule cells.2012

    • Author(s)
      Fujishiro, H., Yano, Y., Takada, Y., Tanihara, M., Himeno, S.
    • Journal Title

      Metallomics

      Volume: 4(7) Issue: 7 Pages: 700-708

    • DOI

      10.1039/c2mt20024d

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 腎臓近位尿細管S1, S2, S3領域由来細胞を用いたシスプラチン毒性の検討.2015

    • Author(s)
      〇藤代瞳、杉本光、姫野誠一郎.
    • Organizer
      日本薬学会135年会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 腎臓近位尿細管におけるカドミウム輸送に関する仮説.2015

    • Author(s)
      〇藤代瞳
    • Organizer
      東京理科大総合研究機構バイオオルガノメタリクス研究部門研究交流会
    • Place of Presentation
      東京
    • Year and Date
      2015-03-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] 腎臓近位尿細管由来細胞を用いたカドミウム輸送系評価システムの検討.2014

    • Author(s)
      〇藤代瞳、高田侑那、矢野悠、姫野誠一郎.
    • Organizer
      第4回メタロミクス研究フォーラム
    • Place of Presentation
      東京
    • Year and Date
      2014-11-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] 132カップ培養システムを用いた消化管および近位尿細管におけるカドミウム輸送機構の解析.2014

    • Author(s)
      〇藤代瞳、濱尾聡子、高田侑那、田中里奈、矢野悠、姫野誠一郎.
    • Organizer
      第4回メタロミクス研究フォーラム、シンポジウムⅡ:異分野融合シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2014-11-05
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] マンガン耐性細胞における乳酸関連遺伝子の発現変化.2014

    • Author(s)
      〇藤代瞳,城公子,姫野誠一郎.
    • Organizer
      フォーラム2014:衛生薬学・環境トキシコロジー
    • Place of Presentation
      筑波
    • Year and Date
      2014-09-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] 腎臓近位尿細管および消化管におけるカドミウム輸送機構.2014

    • Author(s)
      〇藤代瞳、姫野誠一郎.
    • Organizer
      フォーラム2014:衛生薬学・環境トキシコロジー、シンポジウム「金属毒性発現機構解明への新たな戦略」
    • Place of Presentation
      筑波
    • Year and Date
      2014-09-19
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 腎臓近位尿細管由来細胞を用いた新たな毒性・輸送系評価システムの検討.2014

    • Author(s)
      〇藤代瞳、姫野誠一郎.
    • Organizer
      第41回日本毒性学会学術年会、ミニシンポジウム「次世代研究者セミナー~分子毒性学的アプローチと安全性評価~」
    • Place of Presentation
      神戸
    • Year and Date
      2014-07-04
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Analysis of manganese accumulation mechanism by TNFα and IL-1β in SH-SY5Y cells.2014

    • Author(s)
      〇Tomoki Kitayama, Hitomi Fujishiro, Seiichiro Himeno.
    • Organizer
      第24回金属の関与する生体関連反応シンポジウム(SRM2014)
    • Place of Presentation
      京都
    • Year and Date
      2014-06-14 – 2014-06-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] Transport systems of cadmium and manganese in their target organs.2013

    • Author(s)
      ○Hitomi Fujishiro, Seiichiro Himeno
    • Organizer
      第86回日本薬理学会:シンポジウム「カチオントランスポーター研究の新局面」
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] カドミウムおよびマンガン輸送における亜鉛輸送体の役割2013

    • Author(s)
      ○藤代瞳、姫野誠一郎
    • Organizer
      日本薬学会133年会:シンポジウム「メタロミクスの新展開」
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] The roles of metal transporters in target organs of cadmium and manganese.2012

    • Author(s)
      ○藤代瞳、姫野誠一郎
    • Organizer
      第39回日本毒性学会学術年会:シンポジウム「重金属の毒性とその防御の分子メカニズム」
    • Place of Presentation
      仙台
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] カドミウムとマンガンの毒性標的器官における金属輸送体の役割.2012

    • Author(s)
      ○藤代瞳、姫野誠一郎
    • Organizer
      第7回トランスポーター研究会年会:シンポジウム「金属トランスポーターの生物学:メタルが奏でる生命科学の新しい展開」
    • Place of Presentation
      京都
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 消化管におけるカドミウム吸収機構の新たな可能性~DMT1かZIP14か?~2012

    • Author(s)
      ○藤代瞳
    • Organizer
      第31回チョークトーク(生体と金属・化学物質に関する研究会)
    • Place of Presentation
      広島
    • Related Report
      2012 Research-status Report
  • [Presentation] カドミウム輸送の分子機構に関する研究2012

    • Author(s)
      ○藤代瞳
    • Organizer
      フォーラム2012:衛生薬学・環境トキシコロジー:日本薬学会衛生部会・部会賞受賞講演
    • Place of Presentation
      名古屋
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 消化管由来Caco-2細胞でのカドミウム輸送におけるDMT1とZIP14の役割2012

    • Author(s)
      ○藤代瞳、田中里奈、姫野誠一郎
    • Organizer
      フォーラム2012:衛生薬学・環境トキシコロジー
    • Place of Presentation
      名古屋
    • Related Report
      2012 Research-status Report
  • [Presentation] 消化管由来Caco-2細胞におけるカドミウム輸送機構―消化管におけるカドミウム吸収における亜鉛輸送体の役割2012

    • Author(s)
      ○藤代瞳、田中里奈、姫野誠一郎
    • Organizer
      メチル水銀・カドミウム研究ミーティング若手研究発表(環境省)
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] 金属輸送システムとハイブリット分子.

    • Author(s)
      ○藤代瞳、姫野誠一郎
    • Organizer
      第40回日本毒性学会学術年会
    • Place of Presentation
      幕張
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 消化管のカドミウム輸送における金属輸送体の役割.

    • Author(s)
      ○詫間美紀、濱尾聡子、藤代瞳、姫野誠一郎
    • Organizer
      第24回日本微量元素学会
    • Place of Presentation
      大阪
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 123 RBL-2H3細胞由来マンガン耐性細胞を用いた新たなマンガン耐性因子の探索.

    • Author(s)
      ◯藤代瞳,詫間美紀, 大橋俊直, 姫野誠一郎
    • Organizer
      フォーラム2013:衛生薬学・環境トキシコロジー
    • Place of Presentation
      福岡
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 神経細胞におけるマンガン輸送に対する炎症性サイトカインの影響

    • Author(s)
      ○藤代瞳、吉田真梨、姫野誠一郎
    • Organizer
      メタルバイオサイエンス研究会2013
    • Place of Presentation
      静岡
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] マウス腎臓近位尿細管S1, S2, S3領域由来細胞の性状解析

    • Author(s)
      ○藤代瞳、高田侑那、姫野誠一郎
    • Organizer
      メタルバイオサイエンス研究会2013
    • Place of Presentation
      静岡
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 消化管からのカドミウム輸送における金属輸送体の役割.

    • Author(s)
      ○藤代瞳、詫間美紀、姫野誠一郎
    • Organizer
      メタルバイオサイエンス研究会2013
    • Place of Presentation
      静岡
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 乳酸発酵茶の1つである阿波晩茶による血糖値上昇抑制作用.

    • Author(s)
      ◯原田聖大、黒河愛美、秋田浩志、庄司正樹、藤代瞳、日浅未来、江角朋之、葛原隆.
    • Organizer
      日本薬学会133年会
    • Place of Presentation
      熊本
    • Related Report
      2013 Research-status Report
    • Invited
  • [Remarks] 徳島文理大学薬学部HP

    • URL

      http://p.bunri-u.ac.jp/

    • Related Report
      2013 Research-status Report
  • [Remarks] 徳島文理大学薬学部衛生化学講座HP

    • URL

      http://p.bunri-u.ac.jp/lab10/index.html

    • Related Report
      2013 Research-status Report
  • [Remarks] 徳島文理大学薬学部HP

    • URL

      http://p.bunri-u.ac.jp/

    • Related Report
      2012 Research-status Report
  • [Remarks] 徳島文理大学薬学部衛生化学研究室HP

    • URL

      http://p.bunri-u.ac.jp/lab10/index.html

    • Related Report
      2012 Research-status Report
  • [Remarks] 毎日新聞記事掲載

    • URL

      http://p.bunri-u.ac.jp/lab10/12photo.html#mainichi

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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