| Project/Area Number |
24590169
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental pharmacy
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| Research Institution | Sojo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ISHIDA Takumi 崇城大学, 薬学部, 准教授 (10301342)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 酸化ストレス / 糖化反応 / 糖尿病 / 細胞毒性 / 酸化的ストレス |
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| Outline of Final Research Achievements |
Dihydropyrazines (DHPs), formed by nonenzymatic glycation, are interme- diates of the glycation reaction. Since many pyrazine derivatives, which are deduced metabolites of DHPs, have been found in human urine, especially diabetes. In spite of all the available data, the biological effects of DHPs and the effective methods of detection for DHPs in mammalian cells remain elusive. To address this issue, we studied the effects of DHPs on human hepatoma HepG2 cells using DHPs, focusing on oxidative stress by DHPs. DHP-exposed HepG2 cells showed a significant decrease in the ratio of intracellular reduced and oxidized glutathione (GSH/GSSG). In addition, DHP-exposed cells displayed that a number of anti-oxidative stress response genes were distinctively up-regulated. Moreover, DHP-exposed cells also showed the depolymerization of actin, the cell cycle arrest at G2/M phase, and the induction of apoptosis. These findings become a standard for the detection of DHPs in in mammalian cells.
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