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Analysis of the allergenicity change of structurally modified food allergens and development of highly sensitive detection method

Research Project

Project/Area Number 24590171
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental pharmacy
Research InstitutionNational Institute of Health Sciences

Principal Investigator

TESHIMA Reiko  国立医薬品食品衛生研究所, 食品部, 部長 (50132882)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Rhyosuke  国立医薬品食品衛生研究所, 医薬安全科学部, 室長 (50333357)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords食物アレルゲン / 小麦グルテン / 酸加水分解 / 経皮感作 / 交差反応 / 加水分解コラーゲン / 脱アミド化
Outline of Final Research Achievements

We newly obtained following three results in our studies: (1) Increase of IgE production from percutaneous sensitization of BALB/c mice, increase of deamidation of glutamine and increase of molecular weight of some component proteins of wheat gluten after 30-minutes of 0.1N hydrochlonic acid, (2) Increase of reactivity of acid-hydrolyzed wheat protein(HWP) Glupearl 19S-sensitized patient’s IgE to tissue transglutaminase- treated wheat gluten, (3) Development of specific detection method of acid-HWP with monoclonal antibody to specific epitope(QPEEPFPE) of Glupeal 19S.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (49 results)

All 2015 2014 2013 2012 Other

All Journal Article (19 results) (of which Peer Reviewed: 14 results,  Open Access: 3 results) Presentation (29 results) (of which Invited: 9 results) Book (1 results)

  • [Journal Article] RNAi-mediated suppression of endogenous storage proteins leadsto a change in localization of overexpressed cholera toxinB-subunit and the allergen protein RAG2 in rice seeds.2014

    • Author(s)
      Kurokawa, S., et al.
    • Journal Title

      Plant Cell Rep.

      Volume: 33 Issue: 1 Pages: 75-87

    • DOI

      10.1007/s00299-013-1513-3

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential analysis of protein expression in RNA-binding-protein transgenic and parental rice seeds cultivated under salt stress2014

    • Author(s)
      Nakamura R, Nakamura R, Adachi R, Hachisuka A, Yamada A, Ozeki Y, Teshima R.
    • Journal Title

      J Proteome Res.

      Volume: 13 Issue: 2 Pages: 489

    • DOI

      10.1021/pr4006487

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Allergy of Hydrolyzed Wheat Protein via Cutaneous Sensitization.2014

    • Author(s)
      Sakai S, Nakamura R, Nakamura R, Adachi R, Teshima R.
    • Journal Title

      Kagaku To Seibutsu,

      Volume: 52 Pages: 431-437

    • NAID

      130005084897

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Molecular Profile Analysis of Allergenic Hydrolyzed Wheat Protein2014

    • Author(s)
      Sakai S, Adachi R, Nakamura R, Kikuchi H, Watanabe T, Sasaki K, Nishijima K, Ataku H, Nishimaki-Mogami T, Teshima R.
    • Journal Title

      Clinical Immunology & Allergology,

      Volume: 62 Pages: 492-495

    • NAID

      40020265322

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A New Reliable Method for Detecting Specific IgE Antibodies in the Patients with Immediate Type Wheat Allergy due to Hydrolyzed Wheat Protein: Correlation of Its Titer and Clinical Severity2014

    • Author(s)
      Nakamura M., Yagami A.,Hara K., Sano A., Kobayashi T., Aihara M., Hide M.,Chinuki Y., Morita E., Teshima R., Matsunaga K
    • Journal Title

      Allergology International

      Volume: 63 Issue: 2 Pages: 243-249

    • DOI

      10.2332/allergolint.13-OA-0618

    • NAID

      130004477211

    • ISSN
      1323-8930, 1440-1592
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 環境物質と免疫毒性2014

    • Author(s)
      手島 玲子
    • Journal Title

      国立医薬品食品衛生研究所報告

      Volume: 132 Pages: 47-56

    • NAID

      40020342009

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of lactogloblin-specific chimeric human IgEmonoclonal antibodies for in vitro safety assessment of whey hydrolysates.2014

    • Author(s)
      Knipping K., Simons P.J., Buelens-Sleumer L.S., Cox L., den Hartog M., de Jong N., Teshima R., Garssen J., Boon L., Knippels L.M.J.
    • Journal Title

      PLOS ONE

      Volume: 9(8) Issue: 8 Pages: e106025-e106025

    • DOI

      10.1371/journal.pone.0106025

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 経皮感作のメカニズムと食物惹起のクロストーク2014

    • Author(s)
      手島 玲子
    • Journal Title

      日本皮膚アレルギー・接触皮膚炎学会雑誌

      Volume: 8(4) Pages: 249-254

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Low-affinity allergen-specific IgE in cord blood and affinity maturation after birth2014

    • Author(s)
      Kamemura N., Kawamoto N., Nakamura R., Teshima R., Fukao T., Kido H
    • Journal Title

      J. Allergy Clin. Immunol

      Volume: 133 Issue: 3 Pages: 904

    • DOI

      10.1016/j.jaci.2013.09.034

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Food Allergen in Cosmetics2014

    • Author(s)
      Teshima R.
    • Journal Title

      YakugakuZasshi

      Volume: 134(1) Pages: 33-38

    • NAID

      130003382305

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Immunoproteomic analysis of food allergens2014

    • Author(s)
      Nakamura R., Teshima R.
    • Journal Title

      Methods Mol Biol.

      Volume: 1072 Pages: 726-735

    • DOI

      10.1007/978-1-62703-631-3_50

    • ISBN
      9781627036306, 9781627036313
    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] MucoRice-cholera toxin B-subunit, a rice-based oral cholera vaccine, down-regulates the expression of α-amylase/trypsin inhibitor-like protein family as major rice allergens.2013

    • Author(s)
      Kurokawa, S., et al.
    • Journal Title

      J. Proteome Res.

      Volume: 12 Issue: 7 Pages: 3372-3382

    • DOI

      10.1021/pr4002146

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Tissue transglutaminase generates deamidated epitopes on gluten, increasing reactivity with hydrolyzed wheat protein-sensitized IgE2013

    • Author(s)
      Nakamura R, Nakamura R, Sakai S, Adachi R, Hachisuka A, Urisu A, Fukutomi Y, Teshima R.
    • Journal Title

      J. Allergy Clin. Immunol.

      Volume: 132 Issue: 6 Pages: 1436

    • DOI

      10.1016/j.jaci.2013.07.017

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Proteomics-based allergen analysis in plants.2013

    • Author(s)
      Nakamura R., Teshima R.
    • Journal Title

      J. Proteomics

      Volume: 93 Pages: 40-49

    • DOI

      10.1016/j.jprot.2013.03.018

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] 食物アレルゲンの話2013

    • Author(s)
      手島 玲子
    • Journal Title

      日本小児アレルギー学会誌

      Volume: 27 Pages: 15-19

    • NAID

      10031163604

    • Related Report
      2013 Research-status Report
  • [Journal Article] Evaluation of allergenicity of acid-hydrolyzed wheat protein using an in vitro elicitation test2013

    • Author(s)
      Nakamura, R., Nakamura, R., Adachi, R., Itagaki, Y., Fukutomi, Y., Teshima, R
    • Journal Title

      nternational Archives of Allergy and Immunology

      Volume: 160 Issue: 3 Pages: 259

    • DOI

      10.1159/000341671

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 加水分解小麦によるアレルギーについて2013

    • Author(s)
      手島 玲子
    • Journal Title

      ファルマシア

      Volume: 49 Pages: 116-120

    • NAID

      110009995680

    • Related Report
      2012 Research-status Report
  • [Journal Article] アレルゲン特異IgE抗体の新しい測定方法 4.EXiLE法2013

    • Author(s)
      中村亮介、手島玲子
    • Journal Title

      アレルギー・免疫

      Volume: 20 Pages: 62-73

    • Related Report
      2012 Research-status Report
  • [Journal Article] Sensitization to acid-hydrolyzed wheat protein by transdermal administration to BALB/c mice, and comparison with gluten2012

    • Author(s)
      Adachi R., Nakamura R., Sakai S., Fukutomi Y., Teshima R.
    • Journal Title

      Allergy

      Volume: 67 Issue: 11 Pages: 1392

    • DOI

      10.1111/all.12018

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 加水分解コムギ末の抗原解析からわかったこと:今後の対策2015

    • Author(s)
      手島玲子
    • Organizer
      第114回日本皮膚科学学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2015-05-31
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Differential analysis of protein expression in RNA binding protein-transgenic and parental rice seeds cultivated under salt stress and allergenicity test of the rice extracts.2015

    • Author(s)
      Teshima R, Nakamura R., Adachi R., Shindo T., Yamada A., Ohsawa A., Ozeki Y.
    • Organizer
      54rd Society of Toxicology Annual Meeting and ToxExpo
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2015-03-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] 食物アレルギー管理「管理に結びつく基礎的情報」2014

    • Author(s)
      手島玲子
    • Organizer
      日本アレルギー学会第1回総合アレルギー講習会
    • Place of Presentation
      横浜市
    • Year and Date
      2014-12-20
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Experimental Assessments of the Cross-reactivity of IgE from Patients Sensitized with Acid-Hydrolysed Wheat Protein in a Cosmetic Soap.2014

    • Author(s)
      Sakai S, Nakamura R, Adachi R, Fukutomi Y, Saito Y, Mogami T, Teshima R
    • Organizer
      Food Allergy and Anaphylaxis Meeting 2014
    • Place of Presentation
      Dublin, Treland
    • Year and Date
      2014-10-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] 食物アレルゲンの経皮感作による即時型アレルギーモデル2014

    • Author(s)
      安達玲子,酒井信夫,手島玲子
    • Organizer
      第21回日本免疫毒性学会学術年会
    • Place of Presentation
      徳島市
    • Year and Date
      2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] 食品の安全と評価科学2014

    • Author(s)
      手島玲子
    • Organizer
      日本食品化学学会第20回総会・学術大会
    • Place of Presentation
      東京都江東区
    • Year and Date
      2014-05-23
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 経皮感作性を有する加水分解コムギのスクリーニング用抗体について2014

    • Author(s)
      酒井信夫, 安達玲子, 最上知子, 手島玲子
    • Organizer
      日本食品化学学会第20回総会・学術大会
    • Place of Presentation
      東京都江東区
    • Year and Date
      2014-05-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] 抗原性を呈する加水分解コムギの分子プロファイリング2014

    • Author(s)
      酒井信夫, 安達玲子, 木村美恵, 菊地博之, 渡邉敬浩, 佐々木和実, 西嶋桂子, 安宅花子, 福冨友馬, 最上知子, 手島玲子
    • Organizer
      第26回日本アレルギー学会春季臨床大会
    • Place of Presentation
      京都市
    • Year and Date
      2014-05-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] 小麦タンパク質経皮感作能への酸加水分解の効果に関するマウスモデル実験系を用いた検討

    • Author(s)
      安達玲子、酒井信夫、木村美恵、中村里香、福冨友馬、手島玲子
    • Organizer
      第25回日本アレルギー学会春季臨床大会
    • Place of Presentation
      横浜市
    • Related Report
      2013 Research-status Report
  • [Presentation] 小麦グルテンはトランスグルタミナーゼ処理により酸加水分解小麦と同様のIgE反応性を獲得する

    • Author(s)
      中村亮介、中村里香、安達玲子、板垣康治、福富友馬、手島玲子
    • Organizer
      第25回日本アレルギー学会春季臨床大会
    • Place of Presentation
      横浜市
    • Related Report
      2013 Research-status Report
  • [Presentation] 酸加水分解コムギ特異的患者血清IgEはトランスグルタミナーゼ処理小麦グルテンと交差反応する

    • Author(s)
      中村亮介、中村里香、酒井信夫、安達玲子、齋藤嘉朗、宇理須厚雄、福冨友馬、手島玲子
    • Organizer
      第20回日本免疫毒性学会学術大会
    • Place of Presentation
      東京都渋谷区
    • Related Report
      2013 Research-status Report
  • [Presentation] BALB/cマウスにおける酸加水分解コムギタンパク質による経皮感作に関する免疫学的及び病理組織学的解析

    • Author(s)
      曺永晩、 安達玲子、酒井信夫、木村美恵、中村里香、福冨友馬、手島玲子、小川久美子
    • Organizer
      第20回日本免疫毒性学会学術大会
    • Place of Presentation
      東京都渋谷区
    • Related Report
      2013 Research-status Report
  • [Presentation] 加水分解小麦(グルパール19S)に特異的に発現するペプチドの探索及び同定

    • Author(s)
      酒井信夫、中村里香、蓜島由二、福井千恵、鈴木孝昌、中村亮介、蜂須賀暁子、安達玲子、手島玲子
    • Organizer
      第50回全国衛生化学技術協議会年会
    • Place of Presentation
      富山市
    • Related Report
      2013 Research-status Report
  • [Presentation] 小麦グルテンの酸加水分解時間による分子量分布・脱アミド化率の変化

    • Author(s)
      佐々木和実、西嶋桂子、安宅花子、酒井信夫、手島玲子、
    • Organizer
      第43回日本皮膚アレルギー・接触皮膚炎学会学術大会
    • Place of Presentation
      金沢市
    • Related Report
      2013 Research-status Report
  • [Presentation] 加水分解小麦による小麦アレルギー発症の基礎的検討

    • Author(s)
      手島玲子、中村亮介、中村里香、酒井信夫、安達玲子
    • Organizer
      第63回日本アレルギー学会秋季学術大会
    • Place of Presentation
      東京都千代田区
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 加水分解コムギ感作血清中IgEのEXiLE法による検出とその有用性評価

    • Author(s)
      中村亮介、中村政志、矢上晶子、酒井信夫、中村里香、安達玲子、齋藤嘉朗、相原道子、秀道広、千貫祐子、森田栄伸、松永佳世子、手島玲子
    • Organizer
      第63回日本アレルギー学会秋季学術大会
    • Place of Presentation
      東京都千代田区
    • Related Report
      2013 Research-status Report
  • [Presentation] 加水分解コムギによる経皮感作マウスに及ぼす抗原経口負荷の影響

    • Author(s)
      北野高道、山下弘高、安達玲子、手島玲子、福冨友馬、松永佳世子、稲垣直樹、田中宏幸
    • Organizer
      第63回日本アレルギー学会秋季学術大会
    • Place of Presentation
      東京都千代田区
    • Related Report
      2013 Research-status Report
  • [Presentation] ELISA法によるグルパール19S特異IgE抗体評価の有用性評価

    • Author(s)
      中村政志、矢上晶子、相原道子、森田栄伸、秀道広、手島玲子、松永佳世子
    • Organizer
      第63回日本アレルギー学会秋季学術大会
    • Place of Presentation
      東京都千代田区
    • Related Report
      2013 Research-status Report
  • [Presentation] 経皮感作のメカニズムと食物感作のクロストーク

    • Author(s)
      手島玲子
    • Organizer
      第43回日本皮膚アレルギー・接触皮膚炎学会学術大会
    • Place of Presentation
      金沢市
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] コムギタンパク質酸加水分解物の分子特性解析及び経皮感作性の検討

    • Author(s)
      安達玲子,酒井信夫,中村里香,木村美恵,佐々木和実,西嶋桂子,安宅花子,福冨友馬,最上(西巻)知子,手島玲子
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本市
    • Related Report
      2013 Research-status Report
  • [Presentation] Molecular profile analysis of allergenic acid hydrolyzed wheat protein

    • Author(s)
      Sakai S, Adachi R, Nakamura R, Kimura Y, Nakamura R, Sasaki K, Nishijima K, Ataku, H, Fukutomi Y, Nishimaki-Mogami T, Teshima R.
    • Organizer
      53rd Society of Toxicology Annual Meeting and ToxExpo
    • Place of Presentation
      Phoenix, Arizona, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] 酸加水分解小麦のIgE結合性および惹起能の比較検討

    • Author(s)
      中村里香、中村亮介、安達玲子、板垣康治、福富友馬、手島玲子
    • Organizer
      第24回日本アレルギー学会春季臨床大会
    • Place of Presentation
      大阪市
    • Related Report
      2012 Research-status Report
  • [Presentation] 酸加水分解小麦含有石鹸で感作された患者IgEのin vitro活性化試験による交差反応性の評価

    • Author(s)
      中村亮介、中村里香、安達玲子、板垣康治、福富友馬、手島玲子
    • Organizer
      第24回日本アレルギー学会春季臨床大会
    • Place of Presentation
      大阪市
    • Related Report
      2012 Research-status Report
  • [Presentation] 食物アレルゲンの話

    • Author(s)
      手島玲子
    • Organizer
      第49回小児アレルギー学会
    • Place of Presentation
      大阪市
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 酸加水分解小麦含有石鹸に感作された患者血清IgE反応性の解析

    • Author(s)
      中村里香、中村亮介、酒井信夫、安達玲子、板垣康治、福富友馬、手島玲子
    • Organizer
      第19回日本免疫毒性学会学術大会
    • Place of Presentation
      東京都港区
    • Related Report
      2012 Research-status Report
  • [Presentation] 新規食品並びにアレルギー物質の規格と検査について

    • Author(s)
      手島玲子
    • Organizer
      第104回日本食品衛生学会学術講演会
    • Place of Presentation
      岡山市
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Regulation of food containing allergic ingredients in Japan

    • Author(s)
      Teshima R.
    • Organizer
      5th International Fresenius Conference
    • Place of Presentation
      ドイツ、マインツ市
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] EXiLE法による加水分解小麦のアレルゲン性における分子サイズの影響の解析

    • Author(s)
      中村亮介、中村里香、酒井信夫、安達玲子、福富友馬、手島玲子
    • Organizer
      第85回日本生化学会
    • Place of Presentation
      福岡市
    • Related Report
      2012 Research-status Report
  • [Presentation] 化粧品に含まれる食物アレルゲン

    • Author(s)
      手島玲子
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      横浜市
    • Related Report
      2012 Research-status Report
    • Invited
  • [Book] 食物アレルギーの現状とリスク低減化食品素材の開発2015

    • Author(s)
      手島 玲子
    • Total Pages
      299
    • Publisher
      株式会社シーエムシー出版
    • Related Report
      2014 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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