Studies focused on discovery of micro RNA function for biomarkers in chemotherapy against lung cancer

• Project/Area Number: 24590174
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical pharmacy
• Research Institution: Hokkaido University
• Principal Investigator: SHIBAYAMA Yoshihiko 北海道大学, 薬学研究科(研究院), 准教授 (90593822)
• Co-Investigator(Renkei-kenkyūsha): ISEKI Ken 北海道大学, 大学院薬学研究院, 教授 (40203062) ; AKITA Hirotoshi 北海道大学, 大学院医学研究科, 教授 (70222528)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 癌 / マイクロRNA / バイオマーカー / 肺癌 / 大腸癌

Outline of Final Research Achievements

MicroRNA (miRNA) is a non-cording RNA that regulates protein synthesis by mRNA translations, which is critically involved in various physiological processes. We evaluated the relationship among survival in cancer patients, the expression level in patient’s plasma and function of miRNAs. Lower miRNA101-5p expression levels were correlated with a lower probability of survival in lung cancer patient. Lower miRNA124-5p expression levels were also correlated with a lower probability of survival in colon cancer patients. The transfection of mimic miRNA124-5p into cancer cells inhibited cell growth. Our results indicate that expression levels of miRNA101-5p and miRNA124-5p related with survival; expression levels of miRNA101-5p and 124-5p may be used as biomarkers to determine the prognosis of cancer patients.

Research Products

• [Journal Article] Low expression levels of microRNA-124-5p correlated with poor prognosis in colorectal cancer via targeting of SMC4. (2014)
  • Author(s): Jinushi T, Shibayama Y, Kinoshita I, Oizumi S, Jinushi M, Aota T, Takahashi T, Horita S, Dosaka-Akita H, Iseki K.
  • Journal Title: Cancer Medicine Volume: 3 Issue: 6 Pages: 1544-1552
  • DOI: 10.1002/cam4.309
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 肺癌および大腸癌患者における血漿中マイクロRNAの発現：バイオマーカー開発のための探索研究 (2014)
  • Author(s): 柴山良彦、地主隆文、木下一郎、玉木慎也、青田忠博、堀田彰一、佐藤秀紀、、石井理砂、大泉聡史、地主将久、髙﨑雅彦、山田武宏、秋田弘俊、井関健
  • Journal Title: 臨床薬理の進歩2014別刷 Volume: 35 Pages: 106-112
  • Peer Reviewed
• [Presentation] MicroRNA-124* is associated with prognosis in colon cancer via targeting the structural maintenance of chromosomes 4 (2014)
  • Author(s): 柴山良彦、地主隆文、青田忠博、堀田彰一、井関健
  • Organizer: 第12回日本臨床腫瘍学会学術集会
  • Place of Presentation: 福岡市
  • Year and Date: 2014-07-17 – 2014-07-19
• [Presentation] 大腸癌患者における血漿中マイクロRNAの発現：バイオマーカー開発のための探索研究 (2014)
  • Author(s): 柴山良彦、地主隆文、青田忠博、堀田彰一、井関健
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本市(熊本大学）
• [Presentation] 大腸癌患者における血漿中マイクロＲＮＡ発現 (2013)
  • Author(s): 柴山良彦、地主隆文、青田忠博、堀田彰一、井関健
  • Organizer: 第23回日本医療薬学会年会
  • Place of Presentation: 仙台市(仙台国際センター)
• [Presentation] Expression of microRNAs in plasma of patients with colorectal cancer: a screening for a potential biomarker (2013)
  • Author(s): 柴山良彦、地主隆文、青田忠博、堀田彰一、井関健
  • Organizer: 第11回日本臨床腫瘍学会学術集会
  • Place of Presentation: 仙台市(仙台国際センター)