Development of hybrid taste sensor system combined culture of taste bud with voltage sensitive dye

• Project/Area Number: 24590226
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical pharmacy
• Research Institution: Mukogawa Women's University
• Principal Investigator: UCHIDA Takahiro 武庫川女子大学, 薬学部, 教授 (70203536)
• Co-Investigator(Kenkyū-buntansha): YOSHIDA Miyako 武庫川女子大学, 薬学部, 講師 (20369028)
• Co-Investigator(Renkei-kenkyūsha): HAZEKAWA Mai 武庫川女子大学, 薬学部, 助教 (10509186) ; HARAGUCHI Tamami 武庫川女子大学, 薬学部, 助教 (10550741)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: 味蕾細胞 / キニーネ / マクロライド / パターン分析 / 膜電位 / 膜電位感受性色素 / 硫酸キニジン / クラリスロマイシン / フルボキサミン / 精神神経疾患薬 / 5-FU / フロースルーセル

Outline of Final Research Achievements

Cultured taste cell system was developed to evaluate membrane potential -time profile caused by treatment of typical bitterness substance, quinine as an alkaloid, macrolides, and Fluvoxamine. The membrane potential time profiles were monitored for above model substances and its profile was classified. The pattern seemed to be clarified by molecular weight, acidity, and lipophilicity for the targeted substance. The duration of membrane potential suggest after -bitterness of the drug.

Research Products

• [Journal Article] Bitterness evaluation of acidic pharmaceutical substances (NSAIDs) using a taste sensor (2014)
  • Author(s): Yoshida M., Haraguchi T., Uchida T.
  • Journal Title: Chem. Pharm. Bull. Volume: 62 Pages: 1252-1258
  • NAID: 130004712920
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Evaluation of the palatability in 10 different famotidine orally disintegrating tablets by combination of disintegration device and taste sensor (2014)
  • Author(s): Yoshida M., Hazekawa M., Haraguchi T., Uchida T.
  • Journal Title: Drug. Dev. Ind. Pharm Volume: Sep 8 Issue: 8 Pages: 1-6
  • DOI: 10.3109/03639045.2014.954583
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Evaluation of ebastine-loaded orally disintegrating tablets using new apparatus of detecting disintegration time and e-tongue system (2014)
  • Author(s): Haraguchi T., Yoshida M., Uchida T.
  • Journal Title: J. Drug Del. Sci. Tech. Volume: 24 Pages: 684-688
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Evaluation of palatability of 10 commercial amlodipine orally disintegrating tablets by gustatory sensation testing, OD-mate as a new disintegration apparatus and the artificial taste sensor (2013)
  • Author(s): Takahiro Uchida, Miyako Yoshida, Mai Hazekawa, Tamami Haraguchi, Hiroyuki Furuno, Makoto Teraoka, Hidekazu Ikezaki
  • Journal Title: Journal of Pharmacy and Pharmacology Volume: 65 Issue: 9 Pages: 1312-1320
  • DOI: 10.1111/jphp.12101
  • Peer Reviewed
• [Presentation] 小児用製剤の服用性評価 (2015)
  • Author(s): 内田享弘、吉田都、原口珠実、櫨川舞
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 神戸学院大学（兵庫県・神戸市）
  • Year and Date: 2015-03-25 – 2015-03-29
• [Presentation] Screening of refreshments which suppress the bitterness of rebamipide contained in Mucosta ophthalmic suspension UD2% using taste sensor (2013)
  • Author(s): Takahiro Uchida, Tamami Haraguchi, Airi Miyazaki, Miyako Yoshida, Mai Hazekawa
  • Organizer: AAPS annual meeting
  • Place of Presentation: San Antonio, USA
• [Book] Biochemical Sensors: Mimicking Gustatory and Olfactory Senses (2013)
  • Author(s): Takahiro Uchida, Miyako Yoshida
  • Total Pages: 40
  • Publisher: PAN STANFORD PUBLISHING