Roles of Rspo3 during cushion tissue formation in the chick embryonic heart

• Project/Area Number: 24590238
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Osaka City University
• Principal Investigator: YAMAGISHI Toshiyuki 大阪市立大学, 医学(系)研究科(研究院), 講師 (60255122)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 心臓 / 心内膜床 / 発生学 / 発生

Outline of Final Research Achievements

Role of Rspo3 was examined in the endothelial-mesenchymal transformation (EMT) during cushion tissue formation using chick embryonic hearts. First, in situ hybridization showed Rspo3 mRNA was localized during the cushion tissue. Second, in vitro assay for cushion tissue formation showed that the administration of Rspo3, Rspo3+BMP2, and Rspo3+BMP2+wnt11 did not induce cell migration and invasion. Third, chick Rspo1, -2 and -4, genes were cloned and their expression patterns were examined during heart development. These results show that Rspo2 mRNA was expressed in the embryonic heart, but Rspo1 and 4 were not detected.