| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Outline of Final Research Achievements |
Gastrulation is one of important steps for morphogenesis during multicellular animal embryogenesis. However, little is known about their mechanisms. In this study, we investigated the mechanisms and roles of autonomous cell movements in embryonic cells isolated from amphibian gastrula of Japanese newt. Isolated presumptive ectodermal cells carried out mainly circus movement that is based on plasma membrane blebbing. Isolated presumptive mesodermal and endodermal cells, on the other hand, carried out mainly vermiform movement that is based on elongation of cellular body. Their two types of autonomous cell movements in the isolated gastrula cells are regulated by different systems of intracellular calcium signaling and cytoskeleton reassembly. These findings suggest that development and formation of calcium signaling mechanisms depending on a type of germinal layers play an important role in the initiation and execution of morphogenetic cell movements during gastrulation.
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