Morphological analysis of afferent neurons of galanin-like peptide (GALP) using transgenic mice expressing retrogradely transpoted tracer under the control of GALP promoter with Cre-loxP system.
| Project/Area Number |
24590241
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kiryu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SHIODA Seiji 昭和大学, 医学部, 教授 (80102375)
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| Research Collaborator |
HASEGAWA Fumio 昭和大学, 医学部
HIROSE Sachi 昭和大学, 医学部
ITO Kazuo 昭和大学, 医学部
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 神経回路網 / 遺伝子改変動物 / 摂食調節 / ニューロンネットワーク / 求心性ニューロン / Cre-loxPシステム / 多重免疫染色 / ガラニン様ペプチド / 免疫染色 |
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| Outline of Final Research Achievements |
Galanin-like peptide (GALP) plays an important role of feeding regulation and energy metabolism. We have reported on neuronal circuits involving GALP in the hypothalamus. To identify afferent neurons of GALP neurons, we used transgenic mice co-expressing retrogradely transpoted tracer and beta-galactosidase under the control of GALP gene regulatory region using Cre-loxP recombination. Afferent neurons are present in the medial septal, medial habenular nucleus, anterodosal thalamic nucleus, medial preoptic nucleus, paraventricular hypothalamic nucleus, hypothalamic arcuate nucleus and Purkinje cells. These results suggest that GALP neurons function as a regulator of not only feeding and energy metabolism but also several physiological actions such as male-typical sexual behavior and reward system.
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Report
(4 results)
Research Products
(41 results)
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[Journal Article] Neuropeptide W-Induced Hypophagia is Mediated Through Corticotropin-Releasing Hormone-Containing Neurons.2015
Author(s)
Takenoya F, Wang L, Kageyama H, Hirako S, Wada N, Hashimoto H, Ueta Y, Sakagami J, Nonaka, N, Shioda S
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Journal Title
J Mol Neurosci
Volume: in press
Related Report
Peer Reviewed
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[Journal Article] Vagal hyperactivity due to ventromedial hypothalamic lesions increases adiponectin production and release.2014
Author(s)
Suzuki Y, Shimizu H, Ishizuka N, Kubota N, Kubota T, Senoo A, Kageyama H, Osaka T, Hirako S, Kim HJ, Matsumoto A, Shioda S, Mori M, Kadowaki T, Inoue S.
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Journal Title
Diabetes.
Volume: 63(5)
Issue: 5
Pages: 1637-1648
DOI
Related Report
Peer Reviewed
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[Journal Article] Vagal hyperactivity due to ventromedial hypothalamic lesions increases adiponectin production and release.2014
Author(s)
Suzuki Y, Shimizu H, Ishizuka N, Kubota N, Kubota T, Senoo A, Kageyama H, Osaka T, Hirako S, Kim HJ, Matsumoto A, Shioda S, Mori M, Kadowaki T, Inoue S.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Extracellular signal-regulated kinase in the ventromedial hypothalamus mediates leptin-induced glucose uptake in red-type skeletal muscle.2013
Author(s)
Toda C, Shiuchi T, Kageyama H, Okamoto S, Coutinho EA, Sato T, Okamatsu-Ogura Y, Yokota S, Takagi K, Tang L, Saito K, Shioda S, Minokoshi Y.
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Journal Title
Diabetes
Volume: 62
Issue: 7
Pages: 2295-2307
DOI
Related Report
Peer Reviewed
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