Analysis of gradual steps for a specificity formation of cranial placode by using a novel neural plate explant culture.

• Project/Area Number: 24590244
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Jikei University School of Medicine
• Principal Investigator: Shigetani Yasuyo 東京慈恵会医科大学, 医学部, 講師 (70431773)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 神経板 / 神経板外縁 / 神経堤 / 前プラコード外胚葉 / プラコード / BMP4 / FGF2 / 神経板培養 / ニワトリ胚 / ニワトリ / 胚性外胚葉

Outline of Final Research Achievements

A new 'two-step model' has been advocated for the induction of the cranial placode along with the neural crest formation. According to the model, our explanation on the result of the neural plate explant culture should be corrected in part: FGF2 signal possibly has an antagonistic effect on BMP4 signal in our culture system; the induced squamous epithelium has characteristics of an immature composite of the neural border/pre-placodal ectoderm/non-neural ectoderm.

Research Products

• [Presentation] Morphogenesis of the lateral line neuromast in the primitive fish Polypterus. (2015)
  • Author(s): Yasuyo Shigetani, Tohru Yano, and Masataka Okabe
  • Organizer: BMB2015 (第38回 日本分子生物学会年会、第88回 日本生化学会大会 合同大会)
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Presentation] Morphogenesis of the lateral line in the primitive fish Polypterus (2015)
  • Author(s): Yasuyo Shigetani, Tohru Yano, and Masataka Okabe
  • Organizer: 第120回 日本解剖学学会総会・全国学術集会/第92回 日本生理学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Morphogenesis of the lateral line scales in the primitive fish Polypterus (2014)
  • Author(s): Yasuyo Shigetani, Tohru Yano, and Masataka Okabe
  • Organizer: 第37回 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] The vertebrate-specific structures the neural crest and the placed arise from the neural plate border: development of a new culture method for a possible precursor of exterior epithelium of the neural plate. (2013)
  • Author(s): Yasuyo Shigetani, Masataka Okabe
  • Organizer: The 10th International Congress of Vertebrate Morphology 2013
  • Place of Presentation: Barcelona, Spain
• [Presentation] The neural plate cells can be transformed to bordering epithelium of the neural plate (2013)
  • Author(s): Yasuyo Shigetani, Masataka Okabe
  • Organizer: The 23rd CDB Meeting
  • Place of Presentation: 神戸
• [Presentation] The neural plate cells can be transformed to bordering epithelium of the neural plate (2013)
  • Author(s): Yasuyo Shigetani, Masataka Okabe
  • Organizer: CDB Symposium 2013 The Making of a Vertebrate
  • Place of Presentation: 神戸
• [Presentation] WS5b: Development of a new culture method for a precursor to the neural crest and pre-placodal ectoderm (2012)
  • Author(s): Yasuyo Shigetani, Masataka Okabe
  • Organizer: The Joint Meeting of the 45th Annual Meeting of the Japanese Society of Developmental Biologists and the 64th Annual Meeting of the Japan Society for Cell Biologists
  • Place of Presentation: 神戸
  • Invited
• [Presentation] Development of a new culture method for a precursor to the neural crest and pre-placodal ectoderm (2012)
  • Author(s): Yasuyo Shigetani, Masataka Okabe
  • Organizer: The Joint Meeting of the 45th Annual Meeting of the Japanese Society of Developmental Biologists and the 64th Annual Meeting of the Japan Society for Cell Biologists
  • Place of Presentation: 神戸
• [Book] 生命科学概論 環境・エネルギーから医療まで (2012)
  • Author(s): 早稲田大学先進理工学部生命医科学科
  • Total Pages: 158
  • Publisher: 朝倉書店