Intracelluar dynamics of prosaposin using time-lapse recording in cultured cells.
Project/Area Number |
24590254
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General anatomy (including Histology/Embryology)
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Research Institution | Ehime University |
Principal Investigator |
KOBAYASHI Naoto 愛媛大学, 医学(系)研究科(研究院), 教授 (50234836)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | 神経栄養因子 / 神経保護作用 / プロサポシン / プロサポシン受容体 / 培養細胞 / 細胞内動態 / 遺伝子導入 / パーキンソン病 / タイムラプス / 栄養因子 / 細胞培養 / 蛍光色素 |
Outline of Final Research Achievements |
Prosaposin is composed of 524 amino acids, and originally described as the precursor of saposin A, B, C and D, which are responsible to intracellular lipid metabolism. On the other hand, prosaposin is known to show strong neurotrophic activity, and a prosaposin-derived synthetic peptide has neurorotective effect in the central nervous system. Therefore, prosaposin could be a candidate for therapeutic molecule. The present study has revealed intracellular dynamics of prosaposin by its gene induction into cultured cells to chase the traffic route and synthesis/degradation of prosaposin. Degradation of prosaposin has been accerelated, when cultured cells with prosaposin gene induction were treated with MPP+, a drug to establish a model of Parkinsonism. Furthermore, compared with the effect of mock-transfection, prosaposin gene induction has reduced the neurotoxic effects of drugs including kainic acid. These results suggest the prosaposin's potential as a drug for central nervous system.
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Report
(4 results)
Research Products
(24 results)
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[Presentation] Prosaposin and its receptors in the kidney2015
Author(s)
Haruka Watanabe,Tetsuya Shimokawa, Hiroaki Nabeka, Khan MSI, Takuya Doihara, Kimiko Yamamiya, Fumihiko Hamada, Seiji Matsuda, Naoto Kobayashi
Organizer
日本解剖学会総会・全国学術集会
Place of Presentation
神戸市
Year and Date
2015-03-21 – 2015-03-23
Related Report
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[Presentation] Prosaposin and its receptors in the spleen2015
Author(s)
Mitsuaki Takezawa, Tetsuya Shimokawa, Hiroaki Nabeka, Khan MSI, Takuya Doihara, Kimiko Yamamiya, Fumihiko Hamada, Naoto Kobayashi, Seiji Matsuda
Organizer
日本解剖学会総会・全国学術集会
Place of Presentation
神戸市
Year and Date
2015-03-21 – 2015-03-23
Related Report
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