| Project/Area Number |
24590254
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Ehime University |
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Principal Investigator |
KOBAYASHI Naoto 愛媛大学, 医学(系)研究科(研究院), 教授 (50234836)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 神経栄養因子 / 神経保護作用 / プロサポシン / プロサポシン受容体 / 培養細胞 / 細胞内動態 / 遺伝子導入 / パーキンソン病 / タイムラプス / 栄養因子 / 細胞培養 / 蛍光色素 |
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| Outline of Final Research Achievements |
Prosaposin is composed of 524 amino acids, and originally described as the precursor of saposin A, B, C and D, which are responsible to intracellular lipid metabolism. On the other hand, prosaposin is known to show strong neurotrophic activity, and a prosaposin-derived synthetic peptide has neurorotective effect in the central nervous system. Therefore, prosaposin could be a candidate for therapeutic molecule. The present study has revealed intracellular dynamics of prosaposin by its gene induction into cultured cells to chase the traffic route and synthesis/degradation of prosaposin. Degradation of prosaposin has been accerelated, when cultured cells with prosaposin gene induction were treated with MPP+, a drug to establish a model of Parkinsonism. Furthermore, compared with the effect of mock-transfection, prosaposin gene induction has reduced the neurotoxic effects of drugs including kainic acid. These results suggest the prosaposin's potential as a drug for central nervous system.
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