Functional analyses of novel candidate genes involved in axonal guidance obtained by comprehensive genetic screening

• Project/Area Number: 24590256
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: University of Tsukuba (2013-2014); Dokkyo Medical University (2012)
• Principal Investigator: MASUDA TOMOYUKI 筑波大学, 医学医療系, 准教授 (70372828)
• Co-Investigator(Renkei-kenkyūsha): KATO SHIGEKI 福島県立医科大学, 医学部, 講師 (90443879)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 細胞・組織 / 発生・分化 / 神経科学 / 脳・神経 / 神経・組織 / マイクロアレイ

Outline of Final Research Achievements

During development, dorsal root ganglion (DRG) neurons extend their axons both centrally and peripherally. However, the molecular mechanism underlying this phenomenon is less clear. By using in-vivo electroporation and adeno-associated virus vectors, we examined the in-vivo functions of 5 candidate molecules in DRG axonal guidance. Our study revealed the fact that one of the candidate molecules may attract centrally projecting DRG fibers and that the other molecule may attract peripherally projecting DRG ones.

Research Products

• [Journal Article] Congenital diseases and semaphorin signaling: Overview to date of the evidence linking them. (2015)
  • Author(s): Tomoyuki Masuda, Masahiko Taniguchi
  • Journal Title: Congenital Anomalies Volume: 55 Issue: 1 Pages: 26-30
  • DOI: 10.1111/cga.12095
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Attractive and permissive activities of semaphorin 5A toward dorsal root ganglion axons in higher vertebrate embryos (2014)
  • Author(s): MASUDA,T; SAKUMA,C; YAGINUMA,H; TANIGUCHI,M
  • Journal Title: Cell Adhesion ＆ Migration Volume: 8 Issue: 6 Pages: 603-606
  • DOI: 10.4161/19336918.2014.972770
  • NAID: 120007134488
  • Peer Reviewed / Open Access
• [Journal Article] Co-expression of Runx1 and Runx3 in mechanoreceptive dorsal root ganglion neurons. (2013)
  • Author(s): Yoshikawa M, Murakami Y, Senzaki K, Masuda T, Ozaki O, Ito Y, Shiga T.
  • Journal Title: Developmental Neurobiology Volume: 73 Issue: 6 Pages: 469-479
  • DOI: 10.1002/dneu.22073
  • Peer Reviewed
• [Journal Article] Elucidation of target muscle and detailed development of dorsal motor neurons in chick embryo spinal cord. (2013)
  • Author(s): Kobayashi N, Homma S, Okada T, Masuda T, Sato N, Nishiyama K, Sakuma C, Shimada T, Yaginuma H.
  • Journal Title: J Comp Neurol. Volume: in press Issue: 13 Pages: 2987-3002
  • DOI: 10.1002/cne.23326
  • Peer Reviewed
• [Journal Article] Spatiotemporal patterns of the Huntingtin-interacting protein 1-related gene in the mouse head. (2013)
  • Author(s): Masuda T, Sakuma C, Ueno T, Yamada Y, Ohmomo H, Ueda S, Yamagishi T, Yaginuma H.
  • Journal Title: Congenit Anom (Kyoto). Volume: 53 Issue: 4 Pages: 141-148
  • DOI: 10.1111/cga.12023
  • Peer Reviewed
• [Journal Article] Development of the dorsal ramus of the spinal nerve in the chick embryo: A close relationship between development and expression of guidance cues. (2012)
  • Author(s): Masuda T., Sakuma C., Taniguchi M., Kanemoto A., Yoshizawa M., Satomi K., Tanaka H., Takeuchi K., Yaginuma H., Shiga T.
  • Journal Title: Brain Res. Volume: 1480 Pages: 30-40
  • DOI: 10.1016/j.brainres.2012.08.055
  • Peer Reviewed
• [Presentation] 分子生物学的観点からみた脊髄神経後枝の発生：動物実験データからのヒント． (2015)
  • Author(s): 増田知之，志賀隆，八木沼洋行
  • Organizer: 第118回日本解剖学会全国学術集会・第 35 回肉眼解剖学懇話会
  • Place of Presentation: 神戸大学（兵庫県）
  • Year and Date: 2015-03-20
  • Invited
• [Presentation] マウス長鎖遺伝子群の神経系における発現様式とその機能の解析：方法論のパラダイムシフトの現場から． (2014)
  • Author(s): 増田知之，上田秀一，八木沼洋行，谷口雅彦
  • Organizer: 日本解剖学会関東支部第102 回学術集会シンポジウム
  • Place of Presentation: 東京医科大学（東京都）
  • Year and Date: 2014-11-22
  • Invited
• [Presentation] ハンチントン病関連遺伝子Hip1rのマウス頭部における発現様式． (2014)
  • Author(s): 増田知之，山岸敏之，佐久間千恵，小林憲太，大桃秀樹，小林和人，八木沼洋行
  • Organizer: 第119回日本解剖学会全国学術集会
  • Place of Presentation: 自治医科大学キャンパス（栃木県）
  • Year and Date: 2014-03-27
• [Presentation] ハンチントン病モデルマウスにおけるCAGリピートの不安定性とhip1r遺伝子の発現様式の関連性について． (2013)
  • Author(s): 増田知之，佐久間千恵，上野孝之，山岸敏之，大桃秀樹，八木沼洋行，上田秀一
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 国立京都国際会館（京都府）
  • Year and Date: 2013-06-20
• [Presentation] Molecular mechanisms of nerve wiring of the peripheral nervous system. (2013)
  • Author(s): Tomoyuki Masuda, Chie Sakuma, Hiroyuki Yaginuma, Shuichi Ueda
  • Organizer: 第118回日本解剖学会全国学術集会シンポジウム
  • Place of Presentation: 香川県高松市
• [Presentation] Molecular analyses of the development of the spinal nerve in the higher vertebrate embryo. (2012)
  • Author(s): Tomoyuki Masuda, Chie Sakuma, Hiroyuki Yaginuma, Shuichi Ueda
  • Organizer: 14th International Congress of Histochemistry and Cytochemistry
  • Place of Presentation: 京都府京都市
• [Remarks] 増田知之のWebサイト
  • URL: http://www.masudatomoyuki.com/
• [Remarks] 筑波大学研究者総覧
  • URL: http://www.trios.tsukuba.ac.jp/researcher/0000003476
• [Patent(Industrial Property Rights)] AXON ATTRACTANT OR GROWTH PROMOTER, AND METHOD USING THE SAME. (2014)
  • Inventor(s): 増田知之
  • Industrial Property Rights Holder: 国立大学法人 筑波大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-10-09
  • Overseas