| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
It is well known that the changes in morphology of the glomerular epithelium, endothelium, basement membrane and mesangium occur in the diabetic nephropathy. However, the change of these structures at the molecular level is not examined yet. In this study to elucidate the etiology of diabetic nephropathy the change of the molecular structure of glomerulus was examined by the proteomics of O-GlcNAc-modified proteins from isolated glomeruli and immunohistochemistry. It was revealed that in the diabetic glomerulus some proteins including synaptopodin which was expressed specifically in the glomerular epithelial cells increased in O-GlcNAc-modification, and the localization of the O-GlcNAc-modified proteins changed.
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