Analysis on changes in the molecular structure of glomerulus accompanying with diabetic nephropathy
Project/Area Number |
24590260
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General anatomy (including Histology/Embryology)
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Research Institution | Kyorin University |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 糖尿病 / ヘキソサミン代謝 / 糸球体 / O-GlcNAc / 糖尿病性腎症 / 糖転移酵素 / 基底膜 / グライコプロテオミクス / 腎臓 / 糖鎖 / シナプトポディン / アクチン / ガレクチン / 腎症 / 糖鎖修飾 / プロテオミクス / 免疫組織化学 / 細胞培養 / 細胞骨格 / α-アクチニン / GKラット / 質量分析 |
Outline of Final Research Achievements |
It is well known that the changes in morphology of the glomerular epithelium, endothelium, basement membrane and mesangium occur in the diabetic nephropathy. However, the change of these structures at the molecular level is not examined yet. In this study to elucidate the etiology of diabetic nephropathy the change of the molecular structure of glomerulus was examined by the proteomics of O-GlcNAc-modified proteins from isolated glomeruli and immunohistochemistry. It was revealed that in the diabetic glomerulus some proteins including synaptopodin which was expressed specifically in the glomerular epithelial cells increased in O-GlcNAc-modification, and the localization of the O-GlcNAc-modified proteins changed.
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Report
(4 results)
Research Products
(27 results)
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[Presentation] Molecular morphological changes in the glomerulus associated with the elevation of O-GlcNAcylation in diabetic nephropathy.2014
Author(s)
Akimoto Y, Miura Y, Toda T, Fukutomi T, Sugahara D, Wolfert MA, Wells L, Boons G-J, Hart GW, Endo T, Kawakami H
Organizer
The 18th International Microscopy Congress (IMC 2014)
Place of Presentation
Prague, Czech Republic
Year and Date
2014-09-07 – 2014-09-12
Related Report
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[Presentation] Changes of the O-GlcNAc modification of proteins accompanied with diabetic nephropathy.2013
Author(s)
Akimoto Y, Miura Y, Toda T, Fukutomi T, Sugahara D, Wolfert MA, Wells L, Boons G-J, Hart GW, Endo T, Kawakami H
Organizer
12th HUPO World Congress
Place of Presentation
横浜市
Related Report
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[Presentation] Morphological changes in diabetic kidney are associated with increased O-GlcNAc modification of cytoskeletal proteins.2013
Author(s)
Akimoto Y, Miura Y, Toda T, Fukutomi T, Sugahara D, Wolfert MA, Wells L, Boons G-J, Hart GW, Endo T, Kawakami H
Organizer
World Diabetes Congress 2013
Place of Presentation
Melbourne, Australia
Related Report
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