Regulation of hematopoiesis by bone marrow stromal cells in three-dimensional envirnment
Project/Area Number |
24590262
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General anatomy (including Histology/Embryology)
|
Research Institution | Nihon University |
Principal Investigator |
AIZAWA Shin 日本大学, 医学部, 教授 (30202443)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 細胞分化・組織形成 / 造血微小環境 / 骨髄ストローマ細胞 / 三次元培養 / 造血因子 / ストローマ細胞 / 老化促進モデルマウス / 造血幹細胞 / 三次元骨髄培養 / 三次元骨髄培養法 |
Outline of Final Research Achievements |
The proliferation and differentiation of hematopoietic stem cells are regulated by bone marrow microenvironment. Stromal cells, as distinguished from hematopoietic cells, are an essential compartment of this microenvironment. In this study, the stromal cells are shown to regulate the proliferation and differentiation of hematopoietic cells through the mechanism by producing various hematopoietic regulatory factors. Further, a novel in vitro three-dimensional (3D) hematopoietic culture system was developed. In this culture system, stromal cells in 3D culture are stable and regulate hematopoietic cell proliferation and differentiation “quietly” during the culture, resulting in the presence of stable, resting hematopoietic stem cells in 3D culture.
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Report
(4 results)
Research Products
(12 results)