Analysis of unconventional orientation of MaxiK channels in the peri-nuclear ER membrane
Project/Area Number |
24590267
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General physiology
|
Research Institution | Tohoku University |
Principal Investigator |
MURATA Yoshimichi 東北大学, 医学(系)研究科(研究院), 講師 (60455780)
|
Co-Investigator(Renkei-kenkyūsha) |
MARUYAMA Yoshio 東北大学, 大学院医学系研究科, 教授 (00133942)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | イオンチャネル / 小胞体 |
Outline of Final Research Achievements |
We prepared nuclear envelopes (NEs) from HEK293 cells expressing KCa1.1 and observed KCa1.1 currents in the membrane subjected to the patch-clamp technique. The results indicated that the KCa1.1 channels are oriented in the ER membrane as their usual extracellular side faces to cytosol. These observations are inconsistent with the conventional understandings of the protein transport process. Pharmacological and electrophysiological experiments indicated that KCa1.1 channels in our NE preparation didn't oriented bidirectional. They consisted of unconventional orientation. Electrophysiological experiments showed that KCNMB1 wasn't involved in this phenomenon.
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Report
(4 results)
Research Products
(5 results)