Role of transcription factor Elongin A with E3 activity in mouse neural development

• Project/Area Number: 24590279
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General physiology
• Research Institution: Kochi University
• Principal Investigator: YASUKAWA Takashi 高知大学, 教育研究部医療学系, 助教 (60291936)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: Elongin A / 転写伸長因子 / ユビキチンリガーゼ / 神経分化 / 発生

Outline of Final Research Achievements

Elongin A increases the rate of RNA polymerase II (pol II) transcript elongation by suppressing transient pausing by the enzyme. Elongin A also acts as a component of E3 ubiquitin ligase that can target stalled pol II for ubiquitylation and proteasome- dependent degradation. The functions of Elongin A in vivo remain largely unclear. Recently we found that Elongin A-deficient (Elongin A-/-) mouse embryos exhibit abnormalities in the formation of both cranial and spinal nerves and that Elongin A-/- embryonic stem cells show a markedly decreased capacity to differentiate into neurons. Here we dementrated that Elongin A plays a critical role in the development of the sensory nervous system at least in part by regulating the differentiation processes of neural crest cells.

Research Products

• [Journal Article] Transcriptional properties of Mammalian elongin A and its role in stress response. (2013)
  • Author(s): Kawauchi J, Inoue M, Fukuda M, Uchida Y, Yasukawa T, Conaway RC, Conaway JW, Aso T,
  • Journal Title: J Biol Chem. Volume: 288(34) Issue: 34 Pages: 24302-24315
  • DOI: 10.1074/jbc.m113.496703
  • Peer Reviewed
• [Journal Article] Transcriptional elongation factor elongin A regulates retinoic acid-induced gene expression during neuronal differentiation. (2012)
  • Author(s): Yasukawa T, Bhatt S, Takeuchi T, Kawauchi J, Takahashi H, Tsutsui A, Muraoka T, Inoue M, Tsuda M, Kitajima S, Conaway RC, Conaway JW, Trainor PA, Aso T.
  • Journal Title: Cell Rep Volume: 2(5) Issue: 5 Pages: 1129-36
  • DOI: 10.1016/j.celrep.2012.09.031
  • Peer Reviewed
• [Presentation] 転写伸長因子Elongin Aの標的遺伝子の網羅的探索 (2014)
  • Author(s): 安川 孝史, 筒井 文, 村岡 拓也, 佐藤 チエリ, 佐藤 滋生, Ronald C Conaway, Joan W Conaway, 北嶋 繁孝, 麻生 悌二郎
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 感覚神経系の発生・分化におけるElongin Aの標的遺伝子の同定 (2014)
  • Author(s): 安川 孝史, 村岡 拓也, 筒井 文, 佐藤 チエリ, 佐藤 滋生, 川内 潤也, 井上 允, 北嶋 繁孝, Ronald C Conaway, Joan W Conaway, 麻生 悌二郎
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 国立京都国際会館（京都府・京都市）
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] ストレス応答におけるmammalian Elongin Aの特徴と役割 (2014)
  • Author(s): 井上 允, 川内 潤也, 安川 孝史, Ronald C Conaway, Joan W Conaway, 麻生 悌二郎, 北嶋 繁孝
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 国立京都国際会館（京都府・京都市）
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] Role of Transcription Elongation Factor Elongin A in The Sensory Nervous System Development (2012)
  • Author(s): Yasukawa T, Bhatt S, Takeuchi T, Kawauchi J, Takahashi H, Tsutsui A, Muraoka T, Inoue M, Tsuda M, Kitajima S, Conaway RC, Conaway JW, Trainor PA, Aso T
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡 （福岡）
• [Remarks] 高知大学医学部ホームページ お知らせ
  • URL: http://www.kochi-ms.ac.jp/news/24keisai-aso.html
• [Remarks] 高知大学医学部 ホームページ 学部案内 医学科 医学科講座 遺伝子機能解析学
  • URL: http://www.kochi-ms.ac.jp/html/gakubu/fg_chmst.html
• [Remarks] 高知大学医学部遺伝子機能解析学講座ホームページ
  • URL: http://www.kochi-ms.ac.jp/~fg_chmst/index.htm