Effect of preconditioning and postconditioning on ischemic/reperfusion injury

• Project/Area Number: 24590284
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General physiology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: KAMADA Kazuhiro 京都府立医科大学, 医学(系)研究科(研究院), 助教 (80616350)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 虚血再灌流障害 / プレコンディショニング / ポストコンディショニング / 虚血再灌流傷害

Outline of Final Research Achievements

I/R injury is reduced by preconditioning with brief periods of vascular occlusion (ischemic preconditioning: IPC) . Ischemia followed by also more short periods of reperfusion immediately after the ischemia and just before the phase of reperfuion that attenuate postischemic injury (ischemic postconditioning: IPoC). The aim of this study was to evaluate the postischemic antiinflammatory effects of ischemic postconditioning in murine small intestine. Male C57BL/6 mice were using in this study. Mucosal injury and inflammation in the small intestine were evaliated biochemically and histologically. I/R induced severe mucosal injury and neutrophil infiltration. Assessed by tissue associated myeloperoxidase activity. In IPoC group, the post I/R injury in animal small intestine was slightly attenuated compared with I/R group.

Research Products

• [Presentation] マウス小腸虚血再灌流障害における虚血プレコンディショニングおよび虚血ポストコンディショニング効果の検討 (2014)
  • Author(s): 鎌田 和浩
  • Organizer: 第35回日本炎症･再生医学会
  • Place of Presentation: 沖縄
  • Year and Date: 2014-07-01
• [Presentation] Effect of ischemic postconditioning on ischemic/reperfusion injury in murine small intestine
  • Author(s): Kazuhiro Kamada
  • Organizer: 17th Biennial Meeting of Society for Free Radical Research International
  • Place of Presentation: Kyoto, Japan
• [Presentation] マウス小腸虚血再灌流障害における虚血プレコンディショニング、およびポ ストコンディショニング効果の検討
  • Author(s): 鎌田 和浩
  • Organizer: 第35回日本炎症・再生医学会
  • Place of Presentation: 沖縄
• [Presentation] 小腸虚血再灌流モデルにおけるカルシトニン遺伝子関連ペプチド(CGRP)及び一酸化窒素(NO)の関与について
  • Author(s): 鎌田和浩
  • Organizer: 第９回 日本消化管学会総会
  • Place of Presentation: 東京