| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Glycosphingolipids (GSLs) are components of eukaryotic cell membranes. Thousands of different GSLs have been reported due to the structural diversity of their head-group sugar chain and backbone sphingosine base and fatty acid. In the mammalian small intestinal epithelial cells,phytoglycosphingolipids with an additional hydroxyl group at sphinganine C-4.and fatty acid C-2 (t18:0/ hFA)expressed abundantly.In this study,we tried to identify the second enzyme that can produce 2-hydroxy fatty acid in the the small intestinal epithelial cells.In the wild-type mice, the mRNA level of only known fatty acid C-2-hydroxylase (Fa2h) was almost undetectable in the small intestine.In the FA2H deficient mice,2-hydroxy fatty acid containing GSLs were detected in the equal level of wild-type mice.These findings indicate that the second enzyme that can produce 2-hydroxy fatty acid should exist in the small intestinal epithelial cells.
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