Biological role of ceramide structure of glycosphingolipids in the small intestinal epithelial cells
| Project/Area Number |
24590285
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
General physiology
|
|---|
| Research Institution | Kawasaki Medical School (2013-2015)
Tokai University (2012) |
|---|
Principal Investigator |
Matsuda Junko 川崎医科大学, 医学部, 教授 (60363149)
|
|---|
| Project Period (FY) |
2012-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | スフィンゴ糖脂質 / セラミド骨格 / 構造多様性 / 小腸上皮細胞 / 脂肪酸水酸化酵素 / 上皮細胞 / 小腸 / 膜脂質 / セラミド / スフィンゴ脂質 / 生体膜 |
|---|
| Outline of Final Research Achievements |
Glycosphingolipids (GSLs) are components of eukaryotic cell membranes. Thousands of different GSLs have been reported due to the structural diversity of their head-group sugar chain and backbone sphingosine base and fatty acid. In the mammalian small intestinal epithelial cells,phytoglycosphingolipids with an additional hydroxyl group at sphinganine C-4.and fatty acid C-2 (t18:0/ hFA)expressed abundantly.In this study,we tried to identify the second enzyme that can produce 2-hydroxy fatty acid in the the small intestinal epithelial cells.In the wild-type mice, the mRNA level of only known fatty acid C-2-hydroxylase (Fa2h) was almost undetectable in the small intestine.In the FA2H deficient mice,2-hydroxy fatty acid containing GSLs were detected in the equal level of wild-type mice.These findings indicate that the second enzyme that can produce 2-hydroxy fatty acid should exist in the small intestinal epithelial cells.
|
Report
(5 results)
Research Products
(19 results)
