Molecular physiological studies on functional differences among isoforms of ClC-3
| Project/Area Number |
24590291
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | National Institute for Physiological Sciences |
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Principal Investigator |
Okada Toshiaki 生理学研究所, 細胞器官研究系, 特任准教授 (00373283)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | イオンチャネル / 電気生理 / 分子クローニング / 生理学 |
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| Outline of Final Research Achievements |
ClC-3, a member of the Cl-channel/transpoter family, is predicted to have six isoforms, ClC-3a to -3f, with distinct N- and C-terminal amino acid sequences, however amino acid sequences of ClC-3d was derived from genomic sequence, but there was no experimental evidence for mRNAs. We recently cloned a cDNA containing the full coding region of ClC-3d. In this study, we analyzed the channel properties of ClC-3d by whole-cell patch-clamp recordings and identified basic properties of this channel. We identified that ClC-3d, like ClC- 3a, mediates Cd2+-sensitive outwardly rectifying anion currents and that ClC-3d is distinct from the molecular entities of acid- and volume-sensitive anion channels. Electrophysiological properties of ClC-3 have long been investigated but are still unclear. Our results may help elucidate them.
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Report
(5 results)
Research Products
(2 results)
